钙蛋白酶抑制剂对雌激素诱导人乳腺癌细胞上皮-间质转化的影响  

The Effect of Calpain Inhibitor on Estrogen-Induced EMT in Breast Cancer Cells

作　　者：金爱 王宏键 董宇华 王旭东 JIN Ai;WANG Hongjian;DONG Yuhua;WANG Xudong(Department of Physiology,School of Basic Medicine Sciences,Guizhou Medical University,Guiyang 550025,Guizhou,China)

机构地区：[1]贵州医科大学基础医学院生理学教研室,贵州贵阳550025

出　　处：《贵州医科大学学报》2020年第12期1376-1381,共6页Journal of Guizhou Medical University

基　　金：国家自然科学基金资助项目(31660345,31360252)。

摘　　要：目的:探讨钙蛋白酶(Calpain)对17β-雌二醇(E2)诱导人乳腺癌细胞上皮-间质转化(EMT)的影响。方法:以雌激素受体(ER)阳性乳腺癌细胞MCF-7和ER阴性乳腺癌细胞MDA-MB-231细胞为研究模型,分别用二甲基亚砜(DMSO,对照组)、50 nmol/L E2(E2组)及50 nmol/L E2联合10μmol/L Calpeptin(E2+Calpeptin组)处理;采用划痕实验观察各组MCF-7和MDA-MB-231细胞的迁移能力,采用蛋白印迹法检测各组细胞纤连蛋白(FN)和E-钙黏素(E-cadherin)蛋白表达。结果:与对照组比较,E2组MCF-7和MDA-MB-231细胞在24和48 h的迁移率均增加(P<0.01),细胞中的FN蛋白在24、48和72 h表达均明显上调、E-cadherin蛋白表达明显下调(P<0.01);与E2组比较,E2+Calpeptin组MCF-7和MDA-MB-231细胞划痕迁移率明显降低,FN表达明显减少、E-cadherin表达明显增加(P<0.01)。结论:E2可通过Calpain诱导MCF-7和MDA-MB-231乳腺癌细胞的EMT及细胞迁移。Objective:To investigate the effect of calpain on epithelial-mesenchymal transition(EMT)induced by 17β-estradiol(E2).Methods:Estrogen receptor(ER)positive breast cancer cell line MCF-7(ER+)and ER negative breast cancer cell MDA-MB-231(ER-)were used as model cells.Cells were divided into control group(DMSO),E2 group(50 nmol/L),and E2+Calpeptin group(50 nmol/L E2+10μmol/L Calpeptin);then cell migration ability of MCF-7 and MDA-MB-231 cells was observed by wound healing assay,and the expression of fibronectin(FN)and E-cadherin(E-cadherin)was detected by Western blot.Results:Compared with the control group,the migration of MCF-7 and MDA-MB-231 cells were increased significantly in 24 h and 48 h(P<0.01)and the expression of FN was significantly increased and E-cadherin significantly decreased in E2 group at 24 h,48 h and 72 h(P<0.01).Compared with the E2 group,cell migration in E2+Calpeptin group was significantly decreased(P<0.01),while the expression of FN was significantly decreased and E-cadherin was significantly increased(P<0.01).Conclusion:E2 can induce EMT and cell migration of MCF-7 and MDA-MB-231 breast cancer cells via calpain.

关 键 词：细胞运动 17β-雌二醇 钙蛋白酶 乳腺癌细胞 上皮-间质转化 

分 类 号：R737.[医药卫生—肿瘤]