NF-κB表达在胰腺癌的诊断和预后中的价值  

Diagnostic and Prognostic Value of NF-κB Expression Level in Pancreatic Cancer

作　　者：郭江福[1] 范伟[1] 张莹[1] GUO Jiangfu;FAN Wei;ZHANG Ying(Department of Emergency Surgery,Guizhou Provincial People's Hospital,Guiyang 550002,Guizhou,China)

机构地区：[1]贵州省人民医院急诊外科,贵州贵阳550002

出　　处：《贵州医科大学学报》2020年第12期1459-1463,共5页Journal of Guizhou Medical University

基　　金：贵州省科技计划项目[黔科基础2016(1088)]。

摘　　要：目的:探转录因子B(NF-κB)在胰腺癌组织中的表达水平及与肿瘤转移复发的关系。方法:选择手术及术后随访≥24个月、保存完好的胰腺癌样本67例、相应癌旁组织67例以及同期良性胰腺疾病手术样本45例,采用免疫组织化学方法检测3种组织标本中NF-κB表达水平,制作受试者工作特征(ROC)曲线、计算截断值,观察NF-κB水平对胰腺癌诊断的灵敏度、特异度、阳性预测值及符合率;胰腺癌组患者术后随访24月,比较淋巴结及远处转移患者NF-κB表达水平。结果:胰腺癌组织NF-κB表达水平高于癌旁组织、癌旁组织高于良性胰腺疾病组织,差异有统计学意义(P<0.05);NF-κB诊断胰腺癌ROC下曲线面积为0.841,灵敏度、特异度、阳性预测值、符合率分别为91.0%、75.6%、84.7%以及84.8%;胰腺癌淋巴结转移患者的NF-κB表达水平高于无转移患者(P<0.05),术后随访24月时肿瘤扩散、转移、复发患者NF-κB表达水平高于无瘤生存的患者(P<0.05)。结论:NF-κB高表达与胰腺癌复发、转移密切关联。Objective:To explore the expression level of NF-κB transcription factor B in pancreatic cancer tissue and its association with tumor metastasis and recurrence.Methods:Three types of samples including 67 well-preserved pancreatic cancer samples were collected,their corresponding paracancerous tissue samples from patients with postoperative follow-up for≥24 months and 45 benign pancreatic disease surgical samples during the same period.Immunohistochemical(IHC)methods were used to detect NF-κB expression levels for generating receiver operating characteristic(ROC)curve.Cut-off value was calculated.The diagnostic value of NF-κB level in pancreatic cancer were evaluated by observing the sensitivity,specificity,positive predictive value and coincidence rate.The patients were followed up for 24 months after surgery.The expression levels of NF-κB were compared between patients with lymph node and with distant metastasis.Results:The expression level of NF-κB was higher in pancreatic cancer tissue than those in adjacent tissues and benign pancreatic disease tissues(P<0.05).The area of curve(AUC)under ROC for NF-κB diagnosis of pancreatic cancer was 0.841.The sensitivity,the specificity,positive predictive value and coincidence rate were 91.0%,75.6%,84.7%,and 84.8%,respectively.The expression level of NF-κB was higher in patients with lymph node metastasis of pancreatic cancer than that in patients without metastasis(P<0.05).At 24 months after surgery,NF-κB expression level was higher in patients with metastasis and recurrence than those in tumor-free patients(P<0.05).Conclusion:The high expression of NF-κB is closely related to the recurrence and metastasis of pancreatic cancer.

关 键 词：胰腺肿瘤 诊断 复发 淋巴结 免疫组织化学 转录因子NF-ΚB 

分 类 号：R735.9[医药卫生—肿瘤]