盐度渐增对水解微生物群落结构与功能的影响  

Metagenomic Insights into Salinity Build-up in Microbial Communities and Metabolism of Hydrolytic Bioreactor Treating High-color PDWW

作　　者：高京 张少华 王锐[1] 金鹏康[1] GAO Jing;ZHANG Shao-hua;WANG Rui;JIN Peng-kang(School of Environmental and Municipal Engineering,Xi'an University of Architecture and Technology,Xi'an 710055,China)

机构地区：[1]西安建筑科技大学环境与市政工程学院,西安710055

出　　处：《环境科学》2020年第12期5518-5526,共9页Environmental Science

基　　金：国家科技支撑计划项目(2014BAC13B06)。

摘　　要：工业废水循环利用导致盐度渐增,应用水解反应器连续处理硫酸钠盐度渐增的高色度印染废水.在进水盐度从0.5g·L^-1渐增到4 g·L^-1时,印染废水也得以持续高效脱色.宏基因测序表明,盐度渐增导致水解污泥微生物种数从882种下降到了631种,但细菌群落的生物多样性仍保持稳定.盐度渐增对细菌群落的整体功能没有明显影响,但改变了水解脱色功能菌和功能酶的丰度.低盐度条件下Proteobacteria门占主导地位,Methanothrix和Geobacter为参与水解作用的优势属.在盐度渐增时,与众多细菌对盐度胁迫的反应相反,Proteobacteria丰度持续增加,Desulfovibrio和Desulfococcus成为优势属.PICRUSt功能解析显示,脱色酶SOD1和SOD2相对丰度显著下降,CAT和TYR的相对丰度上升,从总体上保证了水解生物系统脱色功能的稳定.本研究从水解脱色功能基因的角度,探讨盐度渐增对水解微生物群落和功能的影响,为盐度渐增胁迫条件下印染废水的脱色和有机物去除机制研究提供理论基础.In this study,to solve the problem of salinity enrichment in industrial wastewater recycling,a hydrolytic bioreactor was continuously operated to treat high-color printing and dyeing wastewater(PDWW)with salinity build-up.Nearly complete color removal was achieved even with salinity build-ups from 0.5 to 4 g·L^-1 in the influent.Pyrosequencing of 16 S rRNA genes showed that the salinity build-up results in the decrease of microbial species from 882 to 631;however,the biodiversity of the bacterial community remains stable.Metagenomic analysis indicated that salinity build-up caused no obvious effect on the overall function of the bacterial community,but altered the abundance of specific decoloring genes.Proteobacteria dominated in the bioreactor,and Methanothrix and Geobacter were the dominant genera under low salinity conditions.Proteobacteria increased in abundance with salinity build-up.Desulfovibrio and Desulfococcus were the two predominant genera in the bioreactor fed with sodium sulphate salinity build-up,demonstrating opposite responses to the sodium stress.PICRUSt functional analysis showed that the relative abundance of the decolorizing enzymes SOD1 and SOD2 decreased significantly,but the relative abundance of CAT and TYR increased,ensuring the stability of the decolorizing function of the hydrolysis biological system.From the perspective of the functional genes of hydrolysis decolorization,this study explored the effect of salinity build-up on the microbial community and function of hydrolysis,providing a theoretical basis for the study of decolorization and organic matter removal mechanism of PDWW under the condition of salinity buildup.

关 键 词：基因组学 盐度胁迫 水解污泥 群落结构 脱色功能 

分 类 号：X172[环境科学与工程—环境科学]