假单胞菌Pseudomonas sp.AT39反式茴脑氧化酶基因(tao)对产茴香酸的影响  

Effects of trans anethole oxidase gene(tao)on anisic acid biosynthesis in Pseudomonas sp.AT39

作　　者：蒋琼[1] 武波[3] 黄罗冬 华燕飞 申佩弘[1,2] JIANG Qiong;WU Bo;HUANG Luodong;HUA Yanfei;SHEN Peihong(University of Guangxi for Life Science and Technology, Guangxi Research Center for Microbial and Enzyme Engineering Technology, Nanning 530004, China;State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Nanning 530004, China;Guangxi Normal University for Nationalities, Chongzuo 532200, China)

机构地区：[1]广西大学生命科学与技术学院,广西微生物与酶工程技术研究中心,广西南宁530004 [2]亚热带农业生物资源保护与利用国家重点实验室,广西南宁530004 [3]广西民族师范学院,广西崇左532200

出　　处：《工业微生物》2020年第6期7-12,共6页Industrial Microbiology

基　　金：广西自然科学基金(2017GXNSFBA198207)。

摘　　要：tao基因编码反式茴脑氧化酶,参与反式茴脑的降解过程。通过自杀质粒pK18mob及广宿主表达质粒pBBR1MCS-5分别构建突变菌AT39/btao、重组菌AT39/tao-pBBR1MCS5及互补菌HAT39/btao。对构建的菌株进行TAO酶比活力测定,结果发现重组菌AT39/tao-pBBR1MCS5的酶比活力显著增加,比野生菌AT39提升了35.23%。茴香酸转化量检测结果显示,重组菌AT39/tao-pBBR1MCS5代谢生成的茴香酸产量为3.55 g/L,同比野生菌AT39增加了约4倍。结果为下一步利用和改造tao基因构建工程菌生产茴香酸奠定了基础。The tao gene encodes trans anethole oxidase and participates in the degradation process of trans anethole.Mutant strain AT39/btao,recombinant strain AT39/tao-pBBR1MCS5 and complementary strain HAT39/btao were constructed by suicide plasmid pK18mob and wide host expression plasmid pBBR1MCS-5,respectively.The TAO enzyme specific activity of the successfully constructed strain was determined.The results showed that the enzyme specific activity of the recombinant strain AT39/tao-pBBR1MCS5 increased by 35.23%compared with that of the wild strain AT39,and the yield of anisic acid produced by the recombinant strain AT39/tao-pBBR1MCS5 was 3.55 g/L,which was an increase of four times compared to that of the wild strain AT39.This research indicated that the tao gene played a key positive regulatory role in the metabolic process of trans-anisine.

关 键 词：假单胞菌AT39 反式茴脑氧化酶基因 茴脑 茴香酸 

分 类 号：R73[医药卫生—肿瘤]