笼目海带多酚的分离纯化及其抗氧化活性研究  

Separation,purification and antioxidant activity of polyphenols from brown alga Kjellmaniella crassifolia Miyabe

作　　者：王帅 郅丽超 梁馨元 吴哲 张梦语 任丹丹 何云海 汪秋宽 WANG Shuai;ZHI Lichao;LIANG Xinyuan;WU Zhe;ZHANG Mengyu;REN Dandan;HE Yunhai;WANG Qiukuan(College of Food Science and Engineering,Key Laboratory of Aquatic Product Processing and Utilization of Liaoning Province,Nation Research and Development Branch Center For Seaweed Processing, Dalian Ocean University,Dalian 116023, China)

机构地区：[1]大连海洋大学食品科学与工程学院,辽宁省水产品加工及综合利用重点实验室,国家海藻加工技术研发分中心,辽宁大连116023

出　　处：《大连海洋大学学报》2020年第6期922-929,共8页Journal of Dalian Ocean University

基　　金：国家重点研发计划蓝色粮仓科技创新专项(2018YFD0901104);辽宁省海洋与渔业厅科研项目(201721);辽宁省教育厅科学研究项目(JL201909);现代农业产业技术体系专项(CARS-50)。

摘　　要：为高效分离纯化笼目海带Kjellmaniella crassifolia Miyabe多酚及其抗氧化活性,采用大孔树脂对笼目海带多酚进行分离纯化,经静态吸附解吸和动态洗脱试验,确定最适分离条件,并通过测定分离组分对DPPH自由基、羟基自由基(·OH)的清除能力、铁离子还原力(FRAP)、氧自由基吸收能力(ORAC),评价其体外抗氧化活性。结果表明:大孔树脂XDA-1为最适分离树脂,其分离最适条件为15 mg/mL的样液以1 mL/min的流速进样100 mL,用70%乙醇洗脱200 mL(2 BV),在此条件下得到两个分离组分P1、P2,其多酚含量分别为粗多酚的15.7倍和3.7倍;通过对各组分的DPPH自由基清除能力、·OH清除能力及FRAP、ORAC值测定发现,笼目海带多酚各组分均有较强的抗氧化活性,其中P2组分表现出较高的抗氧化活性,与多酚其他组分相比,其·OH清除能力、FRAP值和ORAC值具有显著性差异(P<0.05),而P1组分对DPPH自由基具有较好的清除能力,且显著优于粗多酚及P2组(P<0.05)。研究表明,经XDA-1树脂分离纯化的笼目海带多酚均具有较强的抗氧化活性,是天然抗氧化剂的良好来源。Polyphenols were separated and purified from brown alga Kjellmaniella crassifolia Miyabe by macroporous resins and the optimal separation conditions were investigated by static adsorption desorption test and dynamic elution test.The antioxidant activities of polyphenols were determined by various methods including DPPH radical and hydroxyl radical scavenging system,ferric ion reducing antioxidant power and oxygen radical absorbance capacity.The results showed that macroporous resin XDA-1 was the optimal resin for separation,and that the best separation of polyphenols from the brown alga was observed under the optimum conditions of 15 mg/mL of sample concentration,100 mL of sample volume,1 mL/min of rate,70%ethanol of eluent,and 200 mL(2 BV)of elution volume.After elution,two separated components P1 and P2 were obtained,with polyphenol content of 15.7 times of the crude polyphenol in P1,and 3.7 times of the crude polyphenol in P2.The antioxidative tests indicated that all the polyphenols from the brown alga had strong antioxidant activity,P2 showing higher antioxidant activity than other samples(P<0.05)by measuring·OH scavenging ability,FRAP value and ORAC value.There was significant IC50 of scavenging DPPH radicals in P1 compared with other samples(P<0.05),indicating that P1 also had a good antioxidant activity.In conclusion,polyphenols from Kjellmaniella crassifolia Miyabe purified by XDA-1 resin had strong antioxidant activity and were a good source of natural antioxidants.

关 键 词：笼目海带 多酚 分离 抗氧化 

分 类 号：S914.2[农业科学—水产科学]