独行菜抗逆相关转录因子LaDREB密码子偏性与进化分析  

Codon Bias and Evolution Analysis of Stree-Resistant Related Transcription Factor LaDREB in Lepidium apetalum

作　　者：周茜 陈芸 卢函 王玉州 王继莲 赵惠新[2] ZHOU Qian;CHEN Yun;LU Han;WANG Yu-zhou;WANG Ji-lian;ZHAO Hui-xin(College of Life and Geography Science,Key Laboratory of Ecology and Biological Resources in Yarkand Oasis,Kashgar University,Kashi 844006,China;College of Life Science,Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology,Key Laboratory of Plant Stress Biology in Arid Land,College of Life Science,Xinjiang Normal University,Urumqi 830054,China;Hetian Normal College,School of Biology and Geography,Hotan,Xinjiang 848000,China)

机构地区：[1]喀什大学生命与地理科学学院/叶尔羌绿洲生态与生物资源研究高校重点实验室,新疆喀什844000 [2]新疆师范大学生命科学学院/新疆特殊环境物种保护与调控生物学实验室/干旱区植物逆境生物学实验室,新疆乌鲁木齐830054 [3]和田师范专科学校生地学院,新疆和田848000

出　　处：《江西农业大学学报》2020年第6期1237-1249,共13页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金项目(31660079,U1703112);喀什大学校级科研项目(022019110)。

摘　　要：【目的】为明确独行菜抗逆相关转录因子LaDREB基因密码子的使用特性。【方法】运用CodonW与EMBOSS程序及Excel、SPSS、MEGA等软件,分析了独行菜与其他物种中抗逆相关转录因子DREB基因的密码子使用偏好性,比较了独行菜LaDREB基因与模式生物的密码子使用频率。【结果】独行菜LaDREB基因的ENc(有效密码子数)、CAI(密码子适应指数)和GC含量分别为50.49、0.261和45.60%,表明其密码子使用偏性较弱,密码子偏好使用AT结尾;根据RSCU值,确定独行菜LaDREB基因高频密码子11个;不同物种间的DREB基因的密码子存在一定选用偏好性;CDS序列及RSCU聚类分析表明,独行菜LaDREB基因与双子叶植物中十字花科的植物偏好性最相近;密码子碱基成分和相关性分析发现,LaDREB基因在进化过程中密码子偏好性主要受碱基突变压力的影响。【结论】比较密码子使用频率分析发现,原核生物大肠杆菌表达系统更适合作为独行菜LaDREB基因的异源表达受体,模式植物拟南芥、水稻均可作为独行菜LaDREB基因的遗传转化受体,其中拟南芥是其最理想的遗传转化受体。研究为今后独行菜LaDREB基因的异源表达以及相关基因功能的进一步研究提供重要参考。[Objective]To understand the codon bias of stress-resistant related transcription factor LaDREB in Lepidium apetalum Willd.[.Method]CodonW,SPSS,MEGA and EMBOSS online programs and Excel were used to analyze the LaDREB gene codon usage bias of the Lepidium apetalum LaDREB gene.Correlation analysis was conducted to compare the codon usage bias of the Lepidium apetalum LaDREB gene and the DREB genes stress-resistant related transcription factors in other species with those in the model organisms for which relatively mature genetic transformation systems were available[.Result]The ENc,CAI,and GC contents of LaDREB were 50.49,0.261,45.60%,respectively,indicating that the codon bias level of LaDREB in Lepidi⁃um apetalum was low and biased toward the synonymous codons with A or T.According to the RSCU value,11 codons showed high-frequency.Comparison of the codon usage preference of homolongous genes with other crops suggested that there was a certain difference in codon usage preference among DREB genes in different species.The CDS sequence and RSCU cluster analysis showed that the preference of LaDREB gene was the closest to that of crucifers in dicotyledonous plants.The codon base composition and correlation analysis showed that the codon bias of the Lepidium apetalum LaDREB gene had mainly been affected by mutation pressure.[Conclusion]Based on the analysis of the frequency of codon usage across species,the prokaryote E.coli expression system is found to be the most suitable for use with the LaDREB gene,and the model plants Arabidop⁃sis,and tomato can be used as genetic transformation receptors of the LaDREB gene,but Arabidopsis is the most ideal genetic transformation receptor.This study provides important reference information for further studies on the heterologous sexpression and the function of the LaDREB gene in Lepidium apetalum.

关 键 词：独行菜 DREB转录因子 密码子偏性 聚类分析 

分 类 号：Q755[生物学—分子生物学] Q811.4