刺五加苷B对缺氧/复氧诱导的SD大鼠皮层神经元氧化应激损伤的保护作用  

Protective effect of Eleutheroside B on oxidative stress injury induced by hypoxia/reoxygenation in cortical neurons of SD rats

作　　者：赵红晔[1] 邓凤春[2] 沈云虹[1] 金香兰[1] 王滨[1] ZHAO Hong-ye(Department of physiology,Qiqihar medical university,Qiqihar,Heilongjiang,161006,China)

机构地区：[1]齐齐哈尔医学院生理教研室,黑龙江齐齐哈尔161006 [2]齐齐哈尔医学院解剖教研室,黑龙江齐齐哈尔161006

出　　处：《齐齐哈尔医学院学报》2020年第18期2245-2248,共4页Journal of Qiqihar Medical University

基　　金：黑龙江省齐齐哈尔市科学技术计划项目(SFGG-201761)。

摘　　要：目的研究刺五加苷B(EB)对缺氧/复氧诱导的SD大鼠皮层神经元氧化应激损伤的保护作用及其可能的机制。方法原代培养的SD大鼠皮层神经元随机分为Control组、H 4 h/R 24 h组、EB 25μg/ml组和EB 50μg/ml组4组。H 4 h/R 24 h组建立缺氧/复氧模型;EB 25μg/ml组和EB 50μg/ml组先用相应浓度的EB预保护3 h后再造模。采用CCK-8法检测细胞活力,比色法检测乳酸脱氢酶(LDH)活性,荧光探针还原型二氯荧光素探针(DCFH-DA)检测细胞内活性氧(ROS)含量,生化法检测神经元中丙二醛(MDA)的含量以及超氧化物歧化酶(SOD)的活力。结果与Control组相比,H 4 h/R 24 h组神经元细胞活力降低,细胞培养液LDH活性增高,神经元内ROS产生量及MDA含量增高、SOD活力降低(P<0.01)。与H 4 h/R 24 h组相比,EB 25μg/ml组和EB 50μg/ml组神经元细胞活力明显增高,细胞培养液LDH活性明显降低,神经元内ROS产生量及MDA含量显著降低、SOD活力明显升高(P<0.05)。结论在SD大鼠皮层神经元中,EB对缺氧/复氧诱导的细胞氧化应激损伤具有保护作用,可能是通过降低脂质过氧化程度以及提高抗氧化酶系活性实现的。Objective To investigate the protective effect of Eleutheroside B(EB)on oxidative stress injury induced by hypoxia/reoxygenation in cortical neurons of SD rats and its possible mechanism.Methods The primary cultured cortical neurons of SD rats were randomly divided into four groups:control group,H 4 h/R 24 h group,EB 25μg/ml group and EB 50μg/ml group.Hypoxia/reoxygenation model were established in H 4 h/R 24 h group.After pre-protected with the corresponding concentration of EB for 3 h,the neurons were used to establish the Hypoxia/reoxygenation model in EB 25μg/ml group and EB 50μg/ml group.The cell vitality viability of neurons were determined by CCK-8.The activity of lactate dehydrogenase(LDH)in cell culture fluid were determined by colorimetric method.2′,7′-Dichlorofluorescin diacetate(DCFH-DA)was used to detect the generation of reactive oxygen species(ROS)in neurons.The concentration of malondialdehyde(MDA)and the activity of superoxide dismutase(SOD)in neurons were measured by biochemical method.Results Compared with control group,the cell viability of neurons were decreased,the activity of LDH in cell culture fluid were increased,both the generation of ROS and the concentration of MDA in neurons were increased,and the activity of SOD in neurons were decreased in H 4 h/R 24 h group(P<0.01).Compared with H 4 h/R 24 h group,the cell viability of neurons were increased significantly,the activity of LDH in cell culture fluid were remarkably decreased,both the generation of ROS and the content of MDA in neurons were decreased,and the activity of SOD in neurons were increased in EB 25μg/ml group and EB 50μg/ml group(P<0.05).Conclusions EB has a protective effect on oxidative stress injury caused by hypoxia/reoxygenation in cortical neurons of SD rats,which may be related to the reduction of lipid peroxidation and the increase of antioxidant enzyme activity.

关 键 词：刺五加苷B 氧化应激 抗氧化 神经保护 

分 类 号：R743.3[医药卫生—神经病学与精神病学]