竞争性结合的LncRNA和miRNA作为标志物提高宫颈鳞癌患者的诊断和预后  

LncRNAs and miRNAs from a ceRNA Network as a Signature Can Improve the Diagnosis and Prognosis of CESC Patients

作　　者：朱培雯 史学佳 秦士杰 廖敏[1] 马飞[2] Zhu Peiwen;Shi Xuejia;Qin Shijie;Liao Min;Ma Fei(School of Plant Protection,Anhui Agricultural University,Hefei 230036,China;School of Life Sciences,Nanjing Normal University,Nanjing 210023,China)

机构地区：[1]安徽农业大学植物保护学院,安徽合肥230036 [2]南京师范大学生命科学学院,江苏南京210023

出　　处：《南京师大学报（自然科学版）》2020年第4期53-61,共9页Journal of Nanjing Normal University(Natural Science Edition)

基　　金：国家自然科学基金面上项目(31970477)。

摘　　要：宫颈鳞状细胞癌(CESC)严重损害了全世界妇女的健康.因此,寻找新的诊断和预后因子对提高CESC的预防和治疗具有重要价值.为了进一步筛选出有效的生物标志物提高CESC患者的诊断和预后,论文系统地研究了来自癌基因组图谱(TCGA)数据库中的lncRNA、miRNA和mRNA表达情况.研究鉴定了25个lncRNA、14个miRNA和371个mRNA与CESC患者的生存相关;构建了一个与生存相关的lncRNA-miRNA-mRNA竞争性调节网络(ceRNA);发现2个lncRNA(TP73-AS1、CASC15)和3个miRNA(hsa-MiR-142-3p、hsa-MiR-128-3p、hsa-MiR-642a-5p)能够作为CESC的诊断和预后生物标志物,特别是上调的TP73-AS1和CASC15可以作为不良预后因子,而上调的hsa-MiR-142-3p、hsa-MiR-128-3p和hsa-MiR-642a-5p可作为CESC患者生存的良好预后因子.论文研究表明,联合TP73-AS1、CASC15、hsa-MiR-128-3p、hsa-MiR-142-3p和hsa-MiR-642a-5p的预后模型能显著提高CESC患者的预后和可信度.研究不仅为CESC患者确定了几种新颖的诊断和预后因子,而且也揭示了改善CESC患者生存的潜在机制.Cervical squamous cell carcinoma(CESC)seriously harms women’s health worldwide.Therefore,it is of great value to find new diagnostic and prognostic factors to improve the prevention and treatment of CESC.To further screen out effectively biomarkers for promoting the diagnosis and prognosis of CESC patients,it was here systematically investigated the expression landscapes of lncRNAs,miRNAs and mRNAs from The Cancer Genome Atlas(TCGA).In this work,it was firstly identified 25 lncRNAs,14 miRNAs and 371 genes associated with CESC patients’survival.Secondly,it constructed a survival-related lncRNA-miRNA-mRNA ceRNA network.Thirdly,it was discovered that 2 lncRNAs(TP73-AS1 and CASC15),3 miRNAs(hsa-MiR-142-3 p,hsa-MiR-128-3 p and hsa-MiR-642 a-5 p)could be used as promising diagnostic and prognostic biomarkers for CESC,in particular highly expressed TP73-AS1 and CASC15 could act as poor prognostic factors,whereas highly expressed hsa-MiR-142-3 p,hsa-MiR-128-3 p and hsa-MiR-642 a-5 p could serve as good prognostic factors for the survival of CESC patients.Finally,our results demonstrated that a signature containing TP73-AS1,CASC15,hsa-MiR-142-3 p,hsa-MiR-128-3 p and hsa-MiR-642 a-5 p could significantly facilitate the prognosis and credibility of CESC patients.Taken together,our present study identified several novel diagnostic and prognostic factors for CESC patients,and revealed a potential mechanism of facilitating the survival of CESC patients.

关 键 词：宫颈鳞状细胞癌 lncRNA MIRNA ceRNA网络 预后因子 

分 类 号：R737[医药卫生—肿瘤]