转录因子NF-κB1调控前列腺癌中miR-195的表达  

The transcription factor NF-kB1 regulates miR-195 expression in prostate cancer

作　　者：刘春辉 王雅丽 卢凯 张磊 张光远 许斌 陈述求 陈明 LIU Chun-hui;WANG Ya-li;LU Kai;ZHANG Lei;ZHANG Guang-yuan;XU Bin;CHEN Shu-qiu;CHEN Ming(Department of Urology,Zhongda Hospital of Southeast University,Nanjing,Jiangsu 210009,China)

机构地区：[1]东南大学附属中大医院泌尿外科,江苏南京210009

出　　处：《中华男科学杂志》2020年第10期875-880,共6页National Journal of Andrology

基　　金：国家自然科学基金(81572517,81672551,81872089)。

摘　　要：目的:探讨转录因子NF-κB1在前列腺癌中对miR-195表达的调控作用。方法:使用JASPAR数据库分析NF-κB1与miR-195启动子结合的可能性。Oncomine数据库分析NF-κB1在前列腺癌中的表达,通过NF-κB1干扰RNA抑制前列腺癌细胞中NF-κB1的表达,qPCR检测NF-κB1及miR-195的表达。构建miR-195启动子区报告基因质粒,与NF-κB1质粒共转染,检测报告基因活性。通过qPCR检测前列腺癌组织中miR-195和NF-κB1的表达,并分析两者的相关性。结果:NF-κB1在前列腺癌中高表达。在前列腺癌PC-3和DU-145细胞系中抑制NF-κB1的表达后,miR-195的表达升高,且前列腺癌组织中NF-κB1和miR-195的表达呈负相关。荧光素酶报告基因证实,NF-κB1能够与miR-195的启动子区直接结合。结论:转录因子NF-κB1在前列腺癌中调控miR-195的表达。Objective: To investigate the regulatory effect of the transcription factor NF-kB1 on the expression of miR-195 in prostate cancer(PCa). Methods: We analyzed the possibility of NF-kB1 binding to the miR-195 promoter and the expression of NF-kB1 in PCa using the JASPAR and Oncomine databases, respectively, and determined the expressions of NF-kB1 and miR-195 in PCa cells by real-time quantitative PCR after inhibiting the former by interfering RNA targeting NF-kB1. We detected the activity of the luciferase reporter gene after constructing its gene plasmid in the miR-195 promoter region and having it co-transfected with the NF-kB1 plasmid. Then we analyzed the correlation between the expressions of miR-195 and NF-kB1 in the prostate tissue. Results: NF-kB1 was overexpressed in PCa. After inhibition of the expression of NF-kB1, that of miR-195 was increased in PC-3 and DU-145 cell lines, with a negative correlation between the NF-kB1 and miR-195 expressions in the PCa tissue. The results of luciferase reporter gene assay showed direct binding of NF-kB1 to the miR-195 promoter zone. Conclusion: NF-kB1 regulates the expression of miR-195 in prostate cancer. Natl J Androl, 2020, 26(10): 875-880.

关 键 词：NF-κB1 miR-195 前列腺癌 

分 类 号：R737.25[医药卫生—肿瘤]