双重净化法结合液质联用法快速测定鸡蛋中的磺胺类药物残留  

Rapid determination of sulfonamides residues in eggs by dual-cleanup and liquid chromatography-mass spectrometry

作　　者：曲斌[1] 吴玲[1] 刘雨昕 陆桂萍[1] 刘善菁 宋慧敏[1] 强淑萍 QU Bin;WU Ling;LIU Yu-Xin;LU Gui-Ping;LIU Shan-Jing;SONG Hui-Min;QIANG Shu-Ping(Jiangsu Quality Inspection and Testing Center for Animal Products,Nanjing 210036,China;University of South Australia,Adelaide SA5000,Australia)

机构地区：[1]江苏省畜产品质量检验测试中心,南京210036 [2]南澳大学,阿德莱德SA5000

出　　处：《食品安全质量检测学报》2020年第23期8958-8966,共9页Journal of Food Safety and Quality

摘　　要：目的通过双重净化法建立鸡蛋中磺胺类药物残留的液相色谱-质谱检测法。方法样品经乙腈提取,通过式吸附净化和阳离子交换净化,液相色谱分离,四极杆-静电场轨道阱质谱定性定量测定,平行反应监测模式检测,采用内标法定量。结果磺胺类药物保留时间分布在3~7 min,在0.5~20μg/kg范围内呈现良好的线性关系,日内、日间精密度均小于20%,回收率为90%~110%,定量限为0.5μg/kg。结论该方法快速、准确且灵敏度高,为监测磺胺类药物在鸡蛋中的残留监控提供了快速准确的技术手段。Objective To a establish dual-cleanup method for the determination of sulfonamides residues in egg by liquid chromatography-high resolution mass spectrometry. Methods The samples were extracted by acetonitrile,purified by pass-through cleanup and cation exchange solid phase extraction,separated by liquid chromatography,qualitatively and quantitatively determined by quadrupole-orbitrap mass spectrometry,detected by parallel reaction monitoring mode,and quantified by internal standard method. Results The retention time of sulfonamides ranged from 3 and 7 min,and showed a good linear relationship in the range of 0.5 to 20 μg/kg. The precisions were less than 20% between day and day,the recoveries were 90% to 110%,and the limit of quantitative was 0.5 μg/kg. Conclusion The method is rapid,accurate and sensitive,which provides a rapid and accurate technical means for monitoring the residues of sulfonamides in eggs.

关 键 词：磺胺 鸡蛋 残留 液相色谱-质谱联用 

分 类 号：TS253.7[轻工技术与工程—农产品加工及贮藏工程] O657.63[轻工技术与工程—食品科学与工程]