MRI活体示踪PEG/PEI-SPIO标记脂肪源性干细胞移植治疗AD大鼠模型  

In vivo MRI tracking after transplantation of PEG/PEI-SPIO labeled adipose derived stem cells in AD rats models

作　　者：杜磊 雷正贤 汤间仪 马伟琼[4] 谭智霖 廖炎辉 王雅杰 谢琦 DU Lei;LEI Zhengxian;TANG Jianyi;MA Weiqiong;TAN Zhilin;LIAO Yanhui;WANG Yajie;XIE Qi(Department of Medical Imaging, Nansha Hospital, Guangzhou First People's Hospital, the Second Affiliated Hospital of South China University of Technology, Guangzhou 510180, China;Department of Radiology, the University of Hong Kong-Shenzhen Hospital, Shenzhen 518000, China;Department of Radiology, Guangzhou Hospital of Integrated Traditional and West Medicine, Guangzhou 510800, China;Department of Radiology, Huizhou Municipal Central Hospital, Huizhou 516001, China)

机构地区：[1]广州市第一人民医院,华南理工大学附属第二医院南沙医院医学影像科,广东广州510180 [2]香港大学深圳医院放射科,广东深圳518000 [3]广州市中西医结合医院放射科,广东广州510800 [4]惠州市中心人民医院放射科,广东惠州516001

出　　处：《中国介入影像与治疗学》2020年第11期679-684,共6页Chinese Journal of Interventional Imaging and Therapy

基　　金：广东省科技计划项目技术开发类基金(2014A020212034);广州市科信局科学研究专项(1563000424)。

摘　　要：目的观察采用聚乙二醇/聚乙烯亚胺修饰超顺磁性氧化铁(PEG/PEI-SPIO)MRI活体示踪脂肪源性干细胞(ADSC)移植治疗阿尔茨海默病(AD)的可行性。方法将48只AD模型大鼠随机均分为标记组、未标记组和磷酸缓冲盐溶液(PBS)组,分别向侧脑室内注入PEG/PEI-SPIO标记或未标记ADSC及PBS液;另取16只大鼠作为假手术组。分别于第7、14、21、28天行头部MRI,检测海马、颞顶叶皮质、小脑T2值及脑组织铁含量。结果移植后各时间点标记组海马区及皮质区T2值均较其余3组缩短(P均<0.05);第7、14天其皮质区T2值小于海马及小脑,第21、28天海马区T2值小于皮质及小脑(P均<0.05)。相同时间点标记组各脑区铁含量均较其余3组增加(P均<0.05);移植第7、14天,标记组颞顶叶皮质区铁含量较海马及小脑增加(P均<0.05),第21、28天海马区铁含量较颞顶叶皮质及小脑增加(P均<0.05)。结论PEG/PEI-SPIO标记ADSC可反映其在AD模型鼠脑内的分布及迁移,并可通过MRI进行活体示踪。Objective To explore the feasibility of tracking the transplantation of adipose derived stem cells(ADSC)for Alzheimer disease(AD)with polyethylene glycol/polyethyleneimine modified superparamagnetic iron oxide(PEG/PEI-SPIO)with in vivo MRI.Methods Totally 48 AD rat models were randomly divided into labeled group,unlabeled group and phosphate buffer saline(PBS)group(each n=16).PEG/PEI-SPIO labeled or unlabeled ADSC and PBS were injected into the ventricle of rats,respectively.Other 16 rats were taken as the sham operation group.On the 7th,14th,21st and 28th day after transplantation,T2 value of temporal parietal cortex,hippocampus and cerebellum were measured with cranial MRI,and the iron content of brain tissue were measured on the above-mentioned brain areas.Results T2 values of the hippocampus and the cortex were shorter in labeled group than in other groups after transplantation(all P<0.05),and of the cortex were shorter than of the hippocampus and cerebella on the 7th and 14th day,of the hippocampus were shorter than of cortex and cerebella on the 21st and 28th day in the labeled group(all P<0.05).At the same time point,the iron content of the labeled group was higher than that of the other groups in each brain region(all P<0.05).The iron content of the cortex was higher than of other regions in the labeled group on the 7th and 14th day(all P<0.05),and the iron content of the hippocampus was higher than of other regions in the labeled group on the 21st and 28th day(all P<0.05).Conclusion Being labeled with PEG/PEI-SPIO,the distribution and migration of ADSC in the brain of rat AD models could be tracked with in vivo MRI.

关 键 词：阿尔茨海默病 干细胞移植 磁共振成像 大鼠 

分 类 号：R-332[医药卫生] R445.2