房水中IL-17、VEGF、TNF-α水平与先天性白内障摘除术后无晶状体青光眼发生的关系  

The relationship between IL-17,VEGF,TNF-αin aqueous humor and aphakic glaucoma after congenital cataract extraction

作　　者：唐晓蕾[1] 代艳[1] 丁倩[1] 李倩[1] 蒋艳 TANG Xiao-lei;DAI Yan;DING Qian(Department of Ophthalmology,Mianyang Central Hospital,Mianyang 621000,China)

机构地区：[1]绵阳市中心医院眼科,四川绵阳621000

出　　处：《中国处方药》2020年第12期7-9,共3页Journal of China Prescription Drug

摘　　要：目的研究房水中IL-17、VEGF、TNF-α的水平与先天性白内障摘除术后无晶状体青光眼发生的关系。方法选取2016年12月~2018年12月诊治的先天性白内障手术后无晶状体青光眼患者30例(35眼)为青光眼组,先天性白内障术后无青光眼患者30例(42眼)为先天性白内障组。在术后及确诊为青光眼后采集房水,青光眼组进行视野检测以判断视神经损伤情况,术后随访先天性白内障组2年,每6个月采集一次房水,将先天性白内障组分为新生青光眼和非青光眼两个亚组,随访中新诊断为青光眼患者的纳入新生青光眼组。采用双抗体夹心酶联免疫吸附试验(ELISA法)检测房水中IL-17、VEGF、TNF-α的蛋白水平。结果青光眼组根据不同视野损伤程度显示轻度13例、中度16例、重度6例,对应的眼压及IL-17、 VEGF、TNF-α含量比较显示各指标随着损伤程度增加而增加,中度组高于轻度组,重度组高于中度组(P <0.05);房水中IL-17、VEGF、TNF-α水平比较,青光眼组高于先天性白内障组,差异具有统计学意义(P <0.05);两组IL-17与TNF-α具有正相关性(r=0.528、0.948,P <0.05);术后随访显示,先天性白内障组新增青光眼7例11眼,亚组分析新增青光眼患确诊前IL-17、VEGF、TNF-α较未发生青光眼者高,差异具有统计学意义(P<0.05)。Logistic回归分析显示青光眼发生危险同IL-17的高表达正相关(P <0.05)。结论房水中IL-17、VEGF、TNF-α在先天性白内障摘除术后发生无晶状体青光眼患者中高表达,且随着青光眼症状加重而增加表达,其中IL-17是先天性白内障摘除术后发生无晶状体青光眼的危险因素。Objective To study the relationship between the level of IL-17,VEGF,TNF-αin aqueous humor and the occurrence of aphakic glaucoma after congenital cataract extraction.Methods Thirty patients(35 eyes)with aphakic glaucoma after congenital cataract surgery were selected as glaucoma group from December 2016 to December 2018.Thirty patients(42 eyes)without glaucoma after congenital cataract surgery were selected as congenital cataract group.After surgery and after diagnosis of glaucoma,aqueous humor was collected.The congenital cataract group was followed up for 2 years,and aqueous humor was collected every 6 months.The congenital cataract group was divided into two subgroups:neonatal glaucoma and non-glaucoma.During follow-up,the newly diagnosed glaucoma patients were included in the neonatal glaucoma group.Double antibody sandwich enzyme-linked immunosorbent assay(ELISA)was used to detect the level of IL-17,VEGF and TNF-αlpha in aqueous humor.Results According to the degree of visual field injury,13 cases were mild,16 cases were moderate and 6 cases were severe in the glaucoma group.The corresponding intraocular pressure and the content of IL-17,VEGF and TNF-αincreased with the increase of injury degree.The level of intraocular pressure in the moderate group was higher than that in the mild group,and that in the severe group was higher than that in the moderate group(P<0.05);The levels of IL-17,VEGF and TNF-αin aqueous humor were higher in glaucoma group than in congenital cataract group(P<0.05).There was a positive correlation between IL-17 and TNF-αin two groups(r=0.528、0.948,P<0.05).Postoperative follow-up showed that:seven cases(11 eyes)of congenital cataract with new glaucoma were found.Subgroup analysis showed that IL-17,VEGF and TNF-αbefore diagnosis of new glaucoma were higher than those without new glaucoma(P<0.05).Logistic regression analysis showed that the risk of glaucoma was positively correlated with the high expression of IL-17(P<0.05).Conclusion The high expression of IL-17,VEGF and TNF-αin

关 键 词：先天性白内障 无晶状体青光眼 白细胞介素-17 血管内皮生长因子 肿瘤坏死因子-Α 

分 类 号：R779.66[医药卫生—眼科]