Modeling endodermal organ development and diseases using human pluripotent stem cell-derived organoids  

作　　者：Fong Cheng Pan Todd Evans Shuibing Chen 

机构地区：[1]Department of Surgery,Weill Cornell Medical College,New York,NY 10065,USA

出　　处：《Journal of Molecular Cell Biology》2020年第8期580-592,共13页分子细胞生物学报（英文版）

摘　　要：Recent advances in development of protocols for directed differentiation from human pluripotent stem cells(hPSCs)to defined lineages,in combination with 3D organoid technology,have facilitated the generation of various endoderm-derived organoids for in vitro modeling of human gastrointestinal development and associated diseases.In this review,we discuss current state-ofthe-art strategies for generating hPSC-derived endodermal organoids including stomach,liver,pancreatic,small intestine,and colonic organoids.We also review the advantages of using this system to model various human diseases and evaluate the shortcomings of this technology.Finally,we emphasize how other technologies,such as genome editing and bioengineering,can be incorporated into the 3D hPSC-organoid models to generate even more robust and powerful platforms for understanding human organ development and disease modeling.

关 键 词：human pluripotent stem cells ENDODERM gastrointestinal development disease modeling 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]