上皮性卵巢癌组织miR-338-3p、miR-1294的表达及临床意义  

The Expressions and Clinical Significance of miR-338-3p, miR-1294 in Patients with Epithelial Ovarian Cancer

作　　者：徐蕾蕾 华馥 李长华[1] 周树林[2] 徐童 XU Lei-lei;HUA Fu;LI Chang-hua;ZHOU Shu-lin;XU Tong(Department of Gynaecology,Huai'an First Hospital Affiliated to Nanjing Medical University,Huaian,Jiangsu,223001,China;Department of Gynaecology,The First Affiliated Hospital of Nanjing Medical University,Nanjing,Jiangsu,210029,China;Department of Imaging,Huai'an Cancer Hospital,Huaian,Jiangsu,223200,China)

机构地区：[1]南京医科大学附属淮安第一医院妇科,江苏淮安223001 [2]南京医科大学第一附属医院妇科,江苏南京210029 [3]淮安市肿瘤医院影像科,江苏淮安223200

出　　处：《现代生物医学进展》2020年第22期4293-4297,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金面上项目(81472442)。

摘　　要：目的:探讨上皮性卵巢癌组织中微小RNA(miR)-338-3p、miR-1294表达与患者临床病理参数的关系,并分析其表达对上皮性卵巢癌预后的影响。方法:收集2010年6月~2015年6月我院行手术治疗的上皮性卵巢癌患者的石蜡组织样本(癌组织和癌旁正常组织),实时荧光定量PCR(RT-PCR)技术检测组织样本中miR-338-3p、miR-1294的表达情况;分析癌组织中miR-338-3p、miR-1294表达与患者临床病理参数的关系;Kaplan-Meier生存曲线分析miR-338-3p、miR-1294表达与患者预后的关系;Cox回归模型分析上皮性卵巢癌患者的预后影响因素。结果:与癌旁正常组织相比,上皮性卵巢癌组织中miR-338-3p、miR-1294表达均降低(均P<0.05);miR-338-3p表达与组织学分级、淋巴结转移、FIGO分期相关(均P<0.05);miR-1294表达与肿瘤直径、淋巴结转移、FIGO分期相关(均P<0.05)。Kaplan-Meier生存曲线结果显示,miR-338-3p低表达组患者、miR-1294低表达组患者的预后较差(均P<0.05)。Cox回归模型分析结果显示,淋巴结转移、较高FIGO分期、miR-338-3p低表达、miR-1294低表达是上皮性卵巢癌预后的危险因素(均P<0.05)。结论:上皮性卵巢癌组织中miR-338-3p、miR-1294均低表达,且均与较差临床病理参数、预后不良相关;miR-338-3p、miR-1294可能是上皮性卵巢癌患者预后预测的潜在指标。Objective: To explore the relationship between micro RNA(miR)-338-3 p, miR-1294 expression with the clinicopathological parameters in epithelial ovarian cancer tissues, and to analyze their impact on the prognosis in epithelial ovarian cancer. Methods:Paraffin tissue samples(cancer tissue and normal tissue adjacent to cancer) of epithelial ovarian cancer patients in our hospital from June2010 to June 2015 were collected, real-time quantitative PCR(RT-PCR) was used to detect the expression of miR-338-3 p and miR-1294 in tissue samples. The correlation between the miR-338-3 p, miR-1294 expression with the clinicopathological characteristics were analyzed. The Kaplan Meier survival curve was used to analyze the relation of miR-338-3 p, miR-1294 expression with the prognosis of patients. The Cox regression model was used to analyze the influence factors of prognosis. Results: Compared to the adjacent normal tissues, the miR-338-3 p and miR-1294 expression were both down-regulated in the cancer tissues(all P<0.05). The miR-338-3 p expression were related with histological grade, lymph node metastasis and the FIGO stage(all P<0.05). The miR-1294 expression were related with tumor diameter, lymph node metastasis and the FIGO stage(all P<0.05). Kaplan Meier survival curve results showed the prognosis of patients with miR-338-3 p low expression and miR-1294 low expression were poorer(all P<0.05). Cox regression model analysis results showed that the lymph node metastasis, higher FIGO staging, miR-338-3 p low expression and miR-1294 low expression were the risk factors for the prognosis(all P<0.05). Conclusions: In epithelial ovarian cancer, the miR-338-3 p and the miR-1294 are all low expression, which are related to worse clinicopathological parameters and poorer prognosis. The miR-338-3 p and miR-1294 may be potential prognostic indicators for patients with epithelial ovarian cancer.

关 键 词：上皮性卵巢癌 miR-338-3p miR-1294 预后 临床病理参数 

分 类 号：R737.31[医药卫生—肿瘤]