HTLV-1感染T细胞克隆扩增和转化在成人T细胞白血病表达分析  

Expression of HTLV-1-infected T Cell Clone in Adult T Cell Leukemia

作　　者：李玉 熊伟 王三斌 黎诗琪 袁忠涛 LI Yu;XIONG Wei;WANG San-bin;LI Shi-qi;YUAN Zhong-tao(Department of Hematology,the 920 Hospital of the Joint Service of the PLA,Kunming,Yunnan,650032,China;Department of Biochemistry,School of Basic Medical Sciences,Dali University,Dali,Yunnan,671000,China)

机构地区：[1]解放军联勤保障部队第九二〇医院血液科,云南昆明650032 [2]大理大学基础医学院生物化学教研室,云南大理671000

出　　处：《现代生物医学进展》2020年第22期4311-4314,4343,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81560458)。

摘　　要：目的:探究人类T细胞白血病1型病毒(Human T-cell leukemia virus type,HTLV-1)感染的T细胞克隆扩增和转化在成人T细胞白血病(AdultT-cellleukemia,ATL)中的表达分析,探究HTLV-1在T淋巴细胞中发生克隆扩增和转化的机制,为ATL的临床治疗提供理论基础。方法:选择2015年2月至2018年2月于我院接受治疗的38例ATL患者为研究对象,按照其病程差异将其分为急性ATL组(20例)和慢性ALT组(18例),分别采集其血样并检测两组患者血样中HTLV-1病毒载量的差异性,对比两组患者样本中Tax蛋白和HMGB1蛋白的表达情况,并就两组患者血样中肿瘤坏死因子(tumor necrosis factor,TNF-α)、癌胚抗原(carcinoembryonic antigen,CEA)的水平进行对比。结果:(1)急性ATL组患者HTLV-1病毒载量明显高于慢性ATL组患者HTLV-1病毒载量(P<0.05);(2)对比显示,急性ATL组患者血样中Tax蛋白和HMGB1蛋白表达量明显高于慢性ATL组患者(P<0.05);(3)急性ATL组患者中TNF-α和CEA水平均明显高于慢性ATL组患者(P<0.05)。结论:HTLV-1感染ATL患者病程的差异会影响T淋巴细胞的克隆扩增和转化进程,分析其机制可能与HTLV-1能够调控Tax蛋白和HMGB1表达有关。Objective: To explore the expression of human T-cell leukemia virus type 1(HTLV-1) infected T-cell clone amplification and transformation in adult T-cell leukemia(ATL), and to explore the mechanism of HTLV-1 clone amplification and transformation in T-lymphocytes, so as to provide theoretical basis for the clinical treatment of ATL. Methods: 38 ATL patients who were treated in our hospital from February 2015 to February 2018 were selected as the study objects. According to the course difference, they were divided into acute ATL group(20 cases) and chronic ALT group(18 cases). Their blood samples were collected and the differences of HTLV-1 viral load in the blood samples of the two groups were detected. The expression of tax protein and HMGB1 protein in the samples of the two groups were compared the levels of tumor necrosis factor alpha(TNF-α) and carcinoembryonic antigen(CEA) were compared. Results:(1) The viral load of HTLV-1 in acute ATL group was significantly higher than that in chronic ATL group(P<0.05).(2) The expression of tax protein and HMGB1 protein in blood samples in acute ATL group was significantly higher than that in chronic ATL group(P<0.05);(3) The levels of TNF-α and CEA in acute ATL group were significantly higher than those in chronic ATL group(P<0.05).Conclusion: The difference of the course of HTLV-1 infection in patients with ATL will affect the process of T-lymphocyte clone expansion and transformation. The analysis of the mechanism may be related to HTLV-1 regulating the expression of tax protein and HMGB1.

关 键 词：HTLV-1 T细胞 克隆扩增和转化 ATL 表达 

分 类 号：R733.71[医药卫生—肿瘤]