金色链霉菌DM-1全基因组序列测定及去甲金霉素合成基因簇分析  

Genome sequencing of Streptomyces aureofaciens DM-1 and analysis of 6-demethylchlortetracycline biosynthesis gene cluster

作　　者：吴娜新 黄鹤[3] 闵涛玲[1] 胡海峰[1] Naxin Wu;He Huang;Taoling Min;Haifeng Hu(Shanghai Institute of Pharmaceutical Industry,China State Institute of Pharmaceutical Industry,Shanghai 201203,China;School of Pharmacy,Fudan University,Shanghai 201203,China;Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200032,China)

机构地区：[1]中国医药工业研究总院上海医药工业研究院,上海201203 [2]复旦大学药学院,上海201203 [3]中国科学院上海生命科学研究院植物生理与生态研究所,上海200032

出　　处：《生物工程学报》2020年第12期2685-2694,共10页Chinese Journal of Biotechnology

基　　金：国家重大新药创制课题(No.2014ZX09201-001-05);上海市自然科学基金(No.15ZR1440300);上海市产学研合作项目(No.CXY-2013-53)资助。

摘　　要：金色链霉菌Streptomyces aureofaciens DM-1是去甲基金霉素的高产菌株。通过Genome Sequencer FLX系统进行测序,得到一条完整的线性基因组序列,长度为6824334 bp,GC含量为72.6%。结合软件glimmer 3.02、Genemark和Z-Curve program进行基因预测,最终在其基因组中鉴定出6431个基因。应用AntiSMASH软件预测其基因组中存在28个次级代谢生物合成基因簇,其中包含了去甲基金霉素生物合成基因簇。其中甲基转移酶CtcK因移码突变提前终止翻译,很可能是去甲基金霉素相对金霉素(CTC)缺失一个甲基的根本原因。研究结果为S.aureofaciens DM-1的功能基因组学和去甲基金霉素高产菌株育种提供了研究基础。Streptomyces aureofaciens DM-1 is a high-yielding 6-demethylchlortetracycline producer.The genome sequencing of DM-1 reveals a linear chromosome containing 6824334 bps nucleotides with GC content of 72.6%.In this genome,a total of 6431 open reading frames were predicted by using glimmer 3.02,Genemark and Z-Curve softwares.Twenty-eight secondary metabolite biosynthetic gene clusters were uncovered by using AntiSMASH gene prediction software,including the complete 6-demethylchlortetracycline biosynthetic gene cluster.A frame-shift mutation in methyltransferase coding region was detected,which may result in the demethylation of chlortetracycline.The complete genome sequence of S.aureofaciens DM-1 provides basic information for functional genomics studies and selection of high-yielding strains for 6-demethylchlortetracycline.

关 键 词：金色链霉菌 DM-1 基因组测序 去甲基金霉素 生物合成基因簇 

分 类 号：Q933[生物学—微生物学] Q503