基于宏基因组测序的牙周炎患者口腔微生物群落研究  

Study on oral microbial community of periodontitis patients based on macrogenomic sequencing

作　　者：许悦 张宇宸 孙斌 程政[1] 周秦[2] XU Yue;ZHANG Yuchen;SUN Bin;CHENG Zheng;ZHOU Qin(Department of General Dentistry,Faculty of Stomatology,Xi an Jiaotong University,Xi an 710004,China;Department of Implant Dentistry,Xi an Jiaotong University,Xi an 710004,China;Department of Implant Dentistry,Faculty of Stomatology,Tongji University,Shanghai 200072,China)

机构地区：[1]西安交通大学口腔医院综合科,陕西西安710004 [2]西安交通大学口腔医院种植科,陕西西安710004 [3]同济大学附属口腔医院种植科,上海200072

出　　处：《口腔生物医学》2020年第4期242-245,共4页Oral Biomedicine

基　　金：吸烟对种植体周围微生物群落影响的宏基因组学研究(2019SF-144)。

摘　　要：目的:通过宏基因组方法结合高通量测序技术探究牙周炎患者口腔微生物菌群的组成和功能,及其与牙周炎发生发展的相关性。方法:分别采集10名牙周炎患者的唾液和龈下菌斑样本,并提取各样本中微生物总DNA,构建宏基因组文库,进行MGISEQ-2000测序,分别使用Metaphlan2软件、Humann2软件进行生物信息学分析。结果:微生物组成分析结果显示,在属水平上,卟啉单胞菌属在唾液及龈下样本组中的平均相对丰度无统计学差异(P>0.05);普雷沃氏菌属、噬二氧化碳细胞菌属等的相对丰度在两样本组中具有统计学差异(P<0.05)。在种水平上,副流感嗜血杆菌、浅黄奈瑟球菌等在两样本组中相对丰度差异具有统计学意义(P<0.05)。功能分析结果显示,唾液和龈下菌斑样本中贡献菌和序列的相对丰度具有统计学差异(P<0.05)。结论:龈下微生物样本对于牙周炎致病菌方面的研究可能更具代表性,利用宏基因组学研究与牙周炎疾病相关的代谢通路值得进一步发展。Objective:To study the composition and function of the oral microbial flora of patients with periodontitis by metagenomic method combined with high-throughput sequencing technology,and explore the correlation between the composition and function of microorganisms and the development of periodontitis.Methods:Saliva and subgingival plaque samples of 10 patients with periodontitis were collected,and total microbial DNA was extracted from each sample to build the macro-genome library,and MGISeq-2000 sequencing was conducted,while bioinformatics analysis was conducted using Metaphlan2 software and Humann2 software respectively.Results:Among the 20 samples,the microbial composition analysis results showed that at the genus level,the average relative abundance of Porphyromonas in the saliva and subgingival sample groups was not statistically different(P>0.05);the relative abundance of the Prevotella and Capnophagocytes were statistically different between the two sample groups(P<0.05).At the species level,the relative abundance difference of Haemophilus parainfluenzae and Neisseria flavus between the two sample groups was statistically significant(P<0.05).The result of functional analysis showed that the relative abundance of the two groups of contributing bacteria and sequences was statistically different(P<0.05).Conclusions:The results suggested that the subgingival microbe samples may be more representative for the study of periodontitis pathogenic bacteria and that the use of metagenomics to study metabolic pathways related to periodontitis disease may deserve further study.

关 键 词：牙周炎 宏基因组学 高通量测序 口腔微生物 

分 类 号：R781.4[医药卫生—口腔医学]