大肿瘤抑制激酶2在非小细胞肺癌中的表达及临床意义  

Expression and clinical significance of large tumor suppressor kinase 2 in non-small cell lung cancer

作　　者：王红军[1] 贾金广 朱婉凌[1] 魏然[1] 姚菲菲[1] 何炜[2] WANG Hongjun;JIA Jinguang;ZHU Wanling;WEI Ran;YAO Feifei;HE Wei(Zhengzhou People's Hospital,Henan Zhengzhou 450000,China;The First Affiliated Hospital of Zhengzhou University,Henan Zhengzhou 450000,China)

机构地区：[1]郑州人民医院,河南郑州450000 [2]郑州大学第一附属医院,河南郑州450000

出　　处：《现代肿瘤医学》2021年第2期258-262,共5页Journal of Modern Oncology

基　　金：河南省医学科技攻关计划项目(编号:201303021)。

摘　　要：目的:检测大肿瘤抑制激酶2(large tumor suppressor kinase 2,LATS2)在非小细胞肺癌(non-small cell lung cancer,NSCLC)组织和细胞中的表达水平,对NSCLC细胞增殖、侵袭和患者临床特征的影响。方法:收集2017年6月-2019年6月在我院接受手术治疗的NSCLC患者260例,免疫组化实验检测NSCLC和癌旁组织中LATS2的表达水平;卡方检验分析LATS2的表达与患者临床特征的关系;qRT-PCR检测NSCLC组织和癌旁组织中LATS2 mRNA的相对表达水平;转染实验将LATS2质粒转染至A549细胞中;MTT实验检测LATS2对A549细胞增殖的影响;Transwell实验检测LATS2对A549细胞侵袭的影响。结果:LATS2在癌组织中的表达低于癌旁组织;LATS2的高表达率为72.3%(188/260),低表达率为27.7%(72/260);LATS2高表达组与低表达组患者在性别、肿瘤类型、胸膜侵犯、血管侵犯、淋巴管侵犯和T分期之间无统计学差异,在年龄、肿瘤直径、淋巴转移和病理分级之间有统计学差异(P<0.05);NSCLC组织和细胞中LATS2 mRNA的相对表达水平显著低于癌旁组织和人正常肺上皮细胞HPAEpiC;LATS2质粒转染至A549细胞中使其表达水平升高,LATS2高表达组细胞的OD值和发生侵袭的细胞数显著降低。结论:LATS2与患者的年龄、肿瘤直径、淋巴结转移和病理分级有关,其表达水平增加后可显著抑制NSCLC细胞的增殖和侵袭,有可能成为NSCLC治疗的新作用靶点。Objective:To detect the expression of large tumor suppressor kinase 2(LATS2)in non-small cell lung cancer(NSCLC)tissues and cells,and its effect on the proliferation,invasion and clinical characteristics of NSCLC cells.Methods:A total of 260 patients with NSCLC who underwent surgical treatment in our hospital from June 2017 to June 2019 were collected,and the expression level of LATS2 in NSCLC tumor tissues and adjacent tissues was detected by immunohistochemical experiments.Chi-square test was used to analyze the correlation between LATS2 expression and clinical characteristics of patients.qRT-PCR was used to detect the relative expression level of LATS2 mRNA in NSCLC tumor tissues and adjacent tissues.The transfection experiment transfected the LATS2 plasmid into A549 cells.the MTT assay was used to detect the effect of LATS2 on the proliferation of A549 cells.Transwell assay was used to detect the effect of LATS2 on A549 cell invasion.Results:The expression level of LATS2 in tumor tissues was lower than adjacent tissues.The high expression rate of LATS2 was 72.3%(188/260)and the low expression rate was 27.7%(72/260).There was no statistical difference in gender,tumor type,pleural invasion,vascular invasion,lymphatic invasion,and T stage in patients with high expression and low expression in the LATS2 group.There were differences in age,tumor diameter,lymphatic metastasis and pathological grade(P<0.05).The relative expression level of LATS2 mRNA in NSCLC tissues and cells was significantly lower than that of HPAEpiC in adjacent tissues and human normal lung epithelial cells.LATS2 plasmid was transfected into A549 cells to increase its expression level.The OD value and the number of invaded cells in the LATS2 high expression group were significantly reduced.Conclusion:LATS2 is related to the patient's age,tumor diameter,lymph node metastasis and pathological grade.The increased expression level of LATS2 can significantly inhibit the proliferation and invasion of NSCLC cells and may become a new target for the tr

关 键 词：非小细胞肺癌 大肿瘤抑制激酶2 增殖 侵袭 

分 类 号：R734.2[医药卫生—肿瘤]