Snail2、IMP3和Wif-1在胃食管交界腺癌中的表达特点及临床意义  

Expression characteristics and clinical significance of Snail2,IMP3 and Wif-1 in gastroesophageal junction adenocarcinoma

作　　者：姜琳娜[1] 魏娉[1] JIANG Linna;WEI Ping(Department of Pathology,Handan No.1 Hospital,Hebei Handan 056002,China)

机构地区：[1]邯郸市第一医院病理科,河北邯郸056002

出　　处：《现代肿瘤医学》2021年第2期284-288,共5页Journal of Modern Oncology

基　　金：河北省卫计委医学科学研究重点课题(编号:20181641)。

摘　　要：目的:探究Snail2、胰岛素样生长因子II mRNA结合蛋白3(insulin-like growth factor II mRNA-binding protein 3,IMP3)和Wnt通路抑制因子1(Wnt inhibitory factor-1,Wif-1)在食管胃交界处的腺癌(adenocarcinoma of the esophagogastric junction,AEG)中的表达特点及临床意义。方法:随机选择2017年3月至2019年3月间的AEG患者90例作为AEG组,收集同期正常胃组织50例作为健康组。患者经过手术或全身PET-CT检测AEG转移情况。通过免疫组化染色和Western blot检测组织中Snail2、Wif-1、IMP3的表达情况。结果:免疫组化研究结果显示Snail2主要表达于细胞核和细胞质,IMP3主要表达于细胞质,AEG组中Snail2和IMP3的表达水平显著升高。Wif-1在健康组中呈阳性表达,但是在AEG组中表达水平降低。性别、年龄以及肿瘤大小对AEG组织中Snail2、IMP3和Wif-1的影响不大(P>0.05),III/IV分期、出现淋巴转移和远端转移的患者的AEG组织中Snail2、IMP3水平显著增高(P<0.05)。III/IV分期、出现淋巴转移和远端转移的患者的AEG组织中Wif-1水平显著降低(P<0.05)。结论:Snail2和IMP3在AEG中过表达,而Wif-1低表达,并且高水平的Snail2、IMP3和低水平的Wif-1与AEG的恶性特征有关,可作为预测AEG转移、预后和复发的生物标志因子。Objective:To investigate the expression characteristics and clinical significance of Snail2,insulin-like growth factor II mRNA-binding protein 3(IMP3)and Wnt inhibitory factor-1(Wif-1)in adenocarcinoma of gastroesophageal junction(AEG).Methods:Ninety cases of AEG patients from March 2017 to March 2019 were randomly selected as the AEG group,and 50 normal gastric tissues were collected as the healthy group during the same period.Patients were examined for AEG metastases after surgery or whole body PET-CT.The expression of Snail2,Wif-1 and IMP3 in tissues was detected by immunohistochemical staining and Western blot.Results:The results of immunohistochemical studies showed that Snail2 was mainly expressed in the nucleus and cytoplasm.IMP3 was mainly expressed in the cytoplasm.The expression levels of Snail2 and IMP3 in AEG tissues were significantly increased.Wif-1 was positively expressed in healthy tissues,but decreased in AEG tissues.Gender,age,and tumor size had little effect on Snail2,IMP3 and Wif-1 in the AEG group(P>0.05).Patients with stage III/IV,lymphatic metastasis,and distant metastasis had significantly increased Snail2 levels in AEG tissues(P<0.05).Patients with stage III/IV,lymphatic metastasis,and distant metastasis had significantly lower Wif-1 levels in AEG tissues(P<0.05).Conclusion:Snail2 and IMP3 were overexpressed in AEG,while Wif-1 was low expressed,and high levels of Snail2,IMP3 and low levels of Wif-1 were related to the malignant clinical phenotype of AEG,which could be used as biomarkers in prediction of AEG metastasis,prognosis and recurrence.

关 键 词：食管胃交界处的腺癌 Snail2 胰岛素样生长因子II mRNA结合蛋白3 Wnt通路抑制因子1 

分 类 号：R735.1[医药卫生—肿瘤]