烟酰胺单核苷酸对低血糖后葡萄糖再灌注大鼠学习记忆的影响  

Effects of nicotinamide mononucleotide on learning and memory under glucose reperfusion after hypoglycemia in rats

作　　者：王晓楠[1] 徐茜[1] 张励[1] 杨旸[1] 胡雪君[1] WANG Xiao-Nan;XU Xi;ZHANG Li(Department of Gerontology and Geriatrics,the First Affiliated Hospital of China Medical University,Shenyang 110001,Liaoning,China)

机构地区：[1]中国医科大学附属第一医院老年医学科,辽宁沈阳110001

出　　处：《中国老年学杂志》2021年第1期135-138,共4页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助项目(81200246)。

摘　　要：目的探讨研究烟酰胺单核苷酸对低血糖后葡萄糖再灌注大鼠学习记忆能力的影响及其保护作用机制。方法将30只SPF级雄性SD大鼠随机分为假手术组(Sham组,10只)、低血糖后葡萄糖再灌注组(GR组,10只)及低血糖后葡萄糖再灌注并给予烟酰胺单核苷酸治疗组(GR+NMN组,10只)。采用Morris水迷宫实验检测各组大鼠学习记忆能力的变化,免疫荧光方法检测海马组织内NAD^+水平,用2′,7′-二氯二氢荧光素二乙酸酯(H2DCF-DA)来检测海马组织内活性氧(ROS)聚集情况。结果与Sham组比较,GR组逃避潜伏期均显著延长,原平台象限活动时间显著减少,穿越平台次数显著减少,海马组织内NAD^+水平显著降低,海马组织内ROS的水平也显著升高(均P<0.05)。与GR组比较,GR+NMN组,逃避潜伏期均显著缩短;原平台象限活动时间显著增加,穿越平台次数显著增多,海马组织内NAD^+水平显著升高,海马组织内ROS的水平也显著降低(均P<0.05)。结论烟酰胺单核苷酸可显著提高低血糖后葡萄糖再灌注大鼠的学习记忆能力,其作用机制可能与减轻海马组织氧化应激及增加海马组织内NAD^+的水平有关。Objective To investigate the effects and mechanism of nicotinamide mononucleotide(NMN)on learning and memory of under glucose reperfusion after hypoglycemia in rats.Methods A total of 30 rats were randomly divided into sham(n=10),glucose reperfusion after hypoglycemia(GR,n=10),and glucose reperfusion after hypoglycemiaplus NMN(GR+NMN,n=10)groups.The ability of learning and memory was tested by Morris water maze.The NAD+level of hippocampus was measured after its enzymatic conversion to NADH by alcohol dehydrogenase.The reactive oxygen species(ROS)level of hippocampus was measured using a non-fluorescent compound,2',7'-dichlorodihydrofluoresceindiacetate(H2DCF-DA,Invitrogen,USA).Results Compared with sham group,escaping delitescence was significantly prolonged,the activity time on the former platform quadrant significantly reduced,the number of crossing the former platform significantly reduced,the NAD^+ level of hippocampus significantly decreased,and ROS level of hippocampus significantly increased in GR group(all P<0.05).Compared with GR group,escape latency was significantly shortened,the activity time on the former platform quadrant significantly increased,the number of crossing the former platform significantly increased;the neuronal cell death in CA1 of hippocampus significantly decreased,the NAD^+ level of hippocampus significantly restored,and ROS level of hippocampus significantly decreased in GR+NMN group(all P<0.05).Conclusions NMN administration could ameliorate the learning and memory under glucose reperfusion after hypoglycemia,and the mechanism might be related to decreasing ROS formation and restoring the NAD^+ levels.

关 键 词：烟酰胺单核苷酸 低血糖 葡萄糖再灌注 学习记忆能力 氧化应激 

分 类 号：R741[医药卫生—神经病学与精神病学]