亮丙瑞林反加不同剂量雌激素对大鼠子宫异位内膜生长的影响及相关机制研究  

The effect of leuprorelin plus middle or high dose estrogen for treating rats with endometriosis on their ectopic endometrial growth and its mechanism

作　　者：殷金凤[1] 付琼[1] 宋玲 胡婷[1] 熊万春[1] YIN Jinfeng;FU Qiong;SONG Ling;HU Ting;XIONG Wanchun(The Second People’s Hospital of Chengdu,Sichuan Province, 610000)

机构地区：[1]四川省成都市第二人民医院,610000

出　　处：《中国计划生育学杂志》2020年第12期1943-1947,F0002,共6页Chinese Journal of Family Planning

摘　　要：目的:研究亮丙瑞林反添加中高剂量雌激素对大鼠子宫内膜异位内膜生长的影响及相关机制。方法:制备子宫内膜异位症大鼠模型,分为模型组、亮丙瑞林组、低剂量组、中剂量组、高剂量组,每组各15只。模型组生理盐水3 ml灌胃,亮丙瑞林组皮下注射亮丙瑞林1 mg/kg,低、中、高剂量组在亮丙瑞林组给药基础上分别予以戊酸雌二醇25、50、100 g/kg灌胃。分别于给药前后测量异位病灶体积,免疫组织化学法检测组织类固醇激素合成急性调节蛋白(StAR)和肿瘤坏死因子-α(TNF-α)的分布和表达。眼眶取血,采用酶联免疫吸附试验法测定血清糖类抗原125(CA125)。结果:相比模型组,亮丙瑞林组和低、中、高剂量组给药后4、8周后异位病灶体积均缩小,但中、高剂量组给药8周后病灶体积均大于亮丙瑞林组和低剂量组(均P<0.05)。相比模型组,亮丙瑞林组和低、中、高剂量组给药4周后病灶组织StAR、TNF-α表达量下降,给药8周后StAR、TNF-α表达量中、高剂量组高于亮丙瑞林组和低剂量组(均P<0.05)。相比模型组,亮丙瑞林组和低、中、高剂量组给药4周后血清CA125水平下降,中、高剂量组给药8周后血清CA125浓度较亮丙瑞林组和低剂量组升高(均P<0.05)。结论:亮丙瑞林反添加低剂量雌激素(25 g/kg)治疗大鼠子宫内膜异位症效果更好,而反向添加中、高剂量雌激素(50、100 g/kg)可能会促进大鼠子宫异位内膜生长,其相关机制可能与提高了StAR、TNF-α表达及CA125水平有关。Objective:To study the effect of leuprorelin plus middle or high dose estrogen for treating rats with endometriosis on their ectopic endometrial growth,and to explore its mechanism.Methods:The rats with endometriosis were divided into model group,leuprorelin group,estrogen low dose group,estrogen middle dose group,and estrogen high dose group(15 cases in each group).The rats in the model group were given normal saline 3 mL by gavage,the rats in the leuprorelin group were given leuprorelin 1 mg/kg by subcutaneous injection,and the rats in estrogen low dose group,estrogen middle dose group,or estrogen high dose group were given 25,50,or 100 g/kg of estradiol valerate by gavage combined with leuprorelin 1 mg/kg by subcutaneous injection.Before and after administration,the volume of ectopic focus of all rats was measured.The distribution and expression of steroidogenic acute regulatory protein(StAR)and tumor necrosis factor-α(TNF-α)were detected by immunohistochemistry.The level of serum carbohydrate antigen 125(CA125)was measured by enzyme-linked immunosorbent assay.Results:Compared with that of rats in the model group,the volume of lesions of rats in estrogen low dose group,estrogen middle dose group,and estrogen high dose group had decreased significantly at 4 and 8 weeks after administration(P<0.05).Compared with that of rats in leuprorelin group and estrogen low dose group,the volume of lesions of rats in estrogen middle dose group and estrogen high dose had increased significantly at 8 weeks after administration(P<0.05).Compared with those of rats in the model group,the levels of StAR and TNF-αin the lesions tissue of rats in leuprorelin group,and estrogen low dose group,estrogen middle dose group,and estrogen high dose group had decreased significantly(P<0.05).Compared with that of rats in the model group,the serum CA125 level of rats in leuprorelin group,and estrogen low dose group,estrogen middle dose group,and estrogen high dose group had decreased significantly(P<0.05).Compared with that of rats in leup

关 键 词：大鼠子宫内膜异位症 亮丙瑞林 戊酸雌二醇 类固醇激素合成急性调节蛋白 肿瘤坏死因子 

分 类 号：R73[医药卫生—肿瘤]