miR-455-3p靶向HDAC2对卵巢上皮性癌细胞生长和运动的影响  

Effect of miR-455-3p targeting HDAC2 on growth and movement of ovarian epithelial cancer cells

作　　者：杨娟[1] 程国梅[2] 董亚娜[1] 张艳慧[1] 龙文义 Yang Juan;Cheng Guomei;Dong Yana(Medical College of Yellou River University of Science and Technology,Zhengzhou 450063;Dept of Cynecology and 0bstetrics,The Third Affiliated Hospital,Zhengzhou University,Zhengzhou 450052)

机构地区：[1]黄河科技学院医学院,郑州450063 [2]郑州大学第三附属医院妇产科,郑州450052

出　　处：《安徽医科大学学报》2020年第12期1882-1887,共6页Acta Universitatis Medicinalis Anhui

基　　金：2017年河南省科技厅科技发展项目(编号:172102310528)。

摘　　要：目的研究miR-455-3p通过靶向HDAC2对卵巢上皮性癌细胞生长和运动的影响。方法荧光素酶报告实验验证miR-455-3p与HDAC2靶向关系;构建HDAC2pcDNA载体过表达HDAC2,细胞转染mimic,将细胞随机分为4组:Control组、mimic组、HDAC2组、mimic+HDAC2组进行后续实验。BRDU染色检测细胞增殖;流式细胞仪检测细胞凋亡;RT-PCR检测Ki67、PCNA、c-MycmRNA水平;Transwell检测细胞侵袭;划痕试验检测细胞迁移;Westernblot检测血管内皮生长因子(VEGF)、波形蛋白(Vimentin)、纤维粘连蛋白(Fibronectin)蛋白表达水平。结果miR-455-3p和HDAC2存在直接靶向作用关系。与HDAC2组相比较,mim-ic+HDAC2组BRDU阳性细胞数降低(P<0.05),细胞凋亡率升高(P<0.05),Ki67、PCNA、c-MycmRNA水平降低(P<0.05),侵袭细胞数降低(P<0.05),划痕愈合率降低(P<0.05),VEGF、Vimentin、Fibronectin蛋白水平降低(P<0.05)。结论miR-455-3p通过靶向HDAC2诱导卵巢癌细胞HO-8910凋亡,抑制细胞增殖、侵袭和迁移,降低运动能力。Objective To investigate the effect of miR-455-3 p on the growth and movement of ovarian epithelial cancer cells by targeting HDAC2.Methods Luciferase assay was performed to verify the targeting relationship between miR-455-3 p and HDAC2. HDAC2 pcDNA vector was constructed to overexpress HDAC2 and the cells were transfected into mimic. The cells were randomly divided into four groups: Control group, mimic group, HDAC2 group and mimic+HDAC2 group for subsequent experiments. BRDU staining was used to detect cell proliferation. Cell apoptosis was detected by flow cytometry. The mRNA levels of Ki67, PCNA and c-Myc were detected by RT-PCR. Transwell detected cell invasion. Cell migration was detected by scratch test. Western blot detected vascular endothelial growth factor(VEGF), Vimentin and Fibronectin protein expression levels.Results The results showed that miR-455-3 p had directly targets with HDAC2, compared with HDAC2 group, the number of BRDU positive cells in mimic+HDAC2 group significantly reduced(P<0.05), cell apoptosis rate increased significantly(P<0.05), Ki67, PCNA, c-Myc mRNA level decreased significantly(P<0.05), invasive cells significantly reduced(P<0.05), nick healing rate significantly decreased(P<0.05), VEGF, Vimentin and Fibronectin protein levels significantly decreased(P<0.05).Conclusion miR-455-3 p can induce HO-8910 apoptosis of ovarian cancer cells by targeting HDAC2, inhibit cell proliferation, invasion and migration, and reduce movement ability.

关 键 词：卵巢上皮癌 miR-455-3p HDAC2 侵袭和迁移 

分 类 号：R737.31[医药卫生—肿瘤]