STAT3及其多态性在HPV16所致宫颈癌发生过程中的作用研究  

Study on the role of STAT3 and its gene polymorphism on the genesis process of HPV16-induced cervical cancer

作　　者：莫小亮[1] 覃桂荣[1] 蒋晓莉[1] 周卓琳[1] 袁媛[1] MO Xiao-liang;QIN Gui-rong;JIANG Xiao-li;ZHOU Zhuo-lin;YUAN Yuan(Family Planning Ward,the First Affiliated Hospital of Guangxi Medical University,Guangxi Zhuang Autonomous Region,Nanning 530021,China)

机构地区：[1]广西医科大学第一附属医院计划生育病区,广西南宁530021

出　　处：《中国当代医药》2021年第1期16-19,26,共5页China Modern Medicine

基　　金：广西壮族自治区卫生和计划生育委员会自筹经费科研课题(Z2015534)。

摘　　要：目的探究转录活化子3(STAT3)及其基因多态性在人乳头状瘤病毒16(HPV16)感染所致宫颈癌发生过程中的作用。方法抽取2015年1月~2017年12月广西医科大学第一附属医院收治的40例慢性宫颈炎患者、27例宫颈上皮内瘤变患者及17例宫颈癌患者作为研究对象,另外收集10名健康体检者的样本资料,采用聚合酶链反应(PCR)-反向点杂交法、免疫组化检测HPV16 E6蛋白,PCR法检测STAT3表达水平,PCR-制片段长度多态性(RFLP)检测STAT3基因C1697G多态性,对结果进行比较分析。结果宫颈癌患者的E6蛋白阳性表达率高于慢性宫颈炎、宫颈上皮内瘤变患者,差异有统计学意义(P<0.05)。宫颈癌患者的STAT3阳性表达率高于慢性宫颈炎、宫颈上皮内瘤变患者,差异有统计学意义(P<0.05)。宫颈癌、慢性宫颈炎、宫颈上皮内瘤变患者的G/G型、C/C型占比比较,差异有统计学意义(P<0.05);宫颈癌、慢性宫颈炎、宫颈上皮内瘤变患者的G/C型占比比较,差异无统计学意义(P>0.05)。结论STAT3与HPV16所致宫颈癌发生联系紧密,提示STAT3和HPV16在宫颈癌病变发生和发展过程中发挥重要作用。Objective To explore the role of signal transducer and activator of transcription 3(STAT3)and its gene polymorphism on the genesis process of human papillomavirus-16(HPV16)-induced cervical cancer.Methods From January 2015 to December 2017,40 patients with chronic cervicitis,27 patients with cervical intraepithelial neoplasia and 17 patients with cervical cancer admitted to the First Affiliated Hospital of Guangxi Medical University were selected as the research objects,and sample data of 10 health examiners were collected.The polymerase chain reaction(PCR)-reverse dot hybridization and immunohistochemistry were used to detect HPV16 E6 protein,PCR method was used to detect STAT3 expression level,PCR-fragment length polymorphism(RFLP)was used to detect STAT3 gene C1697G polymorphism,and the results were compared and analyzed.Results The positive expression rate of E6 protein in patients with cervical cancer was higher than that in patients with chronic cervicitis and cervical intraepithelial neoplasia,and the difference was statistically significant(P<0.05).The positive expression rate of STAT3 protein in patients with cervical cancer was higher than that in patients with chronic cervicitis and cervical intraepithelial neoplasia,and the difference was statistically significant(P<0.05).There were statistically significant differences in the proportions of G/G and C/C among patients with cervical cancer,chronic cervicitis,and cervical intraepithelial neoplasia(P<0.05).There was no statistically significant difference in the proportion of G/C among patients with cervical cancer,chronic cervicitis,and cervical intraepithelial neoplasia(P>0.05).Conclusion STAT3 has close correlations with HPV16-induced cervical cancer,indicating that STAT3 and HPV16 expressions play an important role in the genesis and development of cervical cancer.

关 键 词：转录活化子3 人乳头状瘤病毒16 E6 宫颈癌 发生过程 

分 类 号：R737[医药卫生—肿瘤]