顶空-气相色谱-质谱联用测定茶叶呋喃类化合物  

Determination of furans in tea by headspace-gas chromatographic mass spectrometry

作　　者：王立媛[1] 应英[1] 胡争艳[1] 王天娇[1] 汤鋆[1] 吴平谷[1] WANG Liyuan;YING Ying;HU Zhengyan;WANG Tianjiao;TANG Jun;WU Pinggu(Zhejiang Provincial Center for Disease Control and Prevention,Hangzhou,Zhejiang 310051,China)

机构地区：[1]浙江省疾病预防控制中心,浙江杭州310051

出　　处：《预防医学》2020年第12期1200-1203,共4页CHINA PREVENTIVE MEDICINE JOURNAL

基　　金：广东省重点领域研发计划项目(2019B020212001)。

摘　　要：目的建立茶叶呋喃类化合物顶空-气相色谱-质谱联用测定方法。方法称取粉碎后的茶叶样品,加入20%氯化钠溶液和同位素内标混合溶液,取顶空气,通过HP-PLOT Q毛细管色谱柱分离,采用电子轰击源(EI)模式的气相色谱-质谱联用测定呋喃、2-甲基呋喃、3-甲基呋喃和2,5-二甲基呋喃。结果在5~400 ng/mL浓度范围内,4种呋喃类化合物的线性关系良好,相关系数为0.999 2~0.999 6。检出限为0.2~1.9μg/kg,定量限为0.4~3.1μg/kg。分别以5.0、20.0、100.0μg/kg加标,平均回收率为95.4%~128.2%,相对标准偏差为0.8%~11.3%。检测5类81份茶叶样品,4种呋喃类化合物含量从高到低依次为红茶、黑茶、乌龙茶、绿茶和花茶。结论该方法顶空前处理装置减少了茶叶的基质干扰,在线性范围、回收率、精密度等方面均能满足检测需求,可同时测定茶叶中4种呋喃类化合物。Objective To establish a method for the determination of furans in tea by headspace-gas chromatographic mass spectrometry. Methods The 20% sodium chloride solution and isotope internal standards were added to the crushed and weighed tea sample. Furan, 2-methylfuran, 3-methylfuran, 2,5-dimethylfuran were separated by HP-PLOT Q capillary column and then determined by gas chromatography mass spectrometry with electron impact ionization mode.Results In the range of 5-400 ng, good linear relationships were observed in the four furan compounds, with the correlation coefficients ranging from 0.999 2 to 0.999 6. The detection limits ranged from 0.2 to 1.9 μg/kg, the quantification limit ranged from 0.4 to 3.1 μg/kg. The recovery rates of furans ranged from 95.4% to 128.2% when spiked at5.0, 20.0 and 100.0 μg/kg, and the relative standard deviations ranged from 0.8% to 11.3%. Eighty-one tea samples were determined, the concentration of four furan compounds was highest in black tea, followed by dark tea, oolong tea,green tea and scented tea. Conclusion Headspace-gas chromatography-mass spectrometry can reduce the matrix interference of tea, and meet the requirements in the linear range, recovery and precision, which is suitable for simultaneous determination of four furan compounds in tea.

关 键 词：顶空-气相色谱-质谱联用 呋喃类化合物 茶叶 

分 类 号：R155[医药卫生—营养与食品卫生学]