白藜芦醇下调NF-κB P65抑制人肺腺癌A549细胞的增殖  

Resveratrol Down-regulates NF-κB P65 and Inhibits Proliferation of Human Lung Adenocarcinoma Cell Line A549

作　　者：刘剑 周继红 黎展华 王雪男 孙桂丽 张北 LIU Jian;ZHOU Jihong;LI Zhanhua;WANG Xuenan;SUN Guili;ZHANG Bei(Guangxi University of Chinese Medicine,Nanning 530001,Guangxi,China;Baoan District Chinese Medicine Hospital,Shenzhen 518104,Guangdong,China;Ruikang Hospital,Guangxi University of Chinese Medicine,Nanning 530001,Guangxi,China)

机构地区：[1]广西中医药大学,广西南宁530001 [2]深圳市宝安区中医院,广东深圳518104 [3]广西中医药大学附属瑞康医院,广西南宁530001

出　　处：《辽宁中医杂志》2020年第11期26-29,I0002,共5页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81760802);广西自然科学基金(2016GXNSFAA380133)。

摘　　要：目的研究白藜芦醇(resveratrol,Res)通过下调核因子κB(nuclear factor kappa-B,NF-κB)P65对人肺腺癌A549细胞增殖的影响。方法使用细胞计数试剂盒-8(CCK-8)测定A549细胞的增殖;选用逆转录-聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)检测Res对A549细胞NF-κB P65 mRNA表达的影响;采用流式细胞仪分析A549细胞的细胞周期及凋亡;用蛋白免疫印迹法(Western blotting)检测A549细胞NF-κB P65蛋白的表达。结果 Res可通过下调NF-κB P65抑制人肺腺癌A549细胞的增殖,阻止A549细胞周期、促进细胞凋亡,同时抑制其蛋白水平的表达。结论 Res通过下调NF-κB P65可以明显抑制人肺腺癌A549细胞的增殖。Objective Resveratrol(Res) was used to down-regulate the proliferation of human lung adenocarcinoma A549 cells by down-regulating nuclear factor kappa B(NF-κB)P65.Methods The proliferation of A549 cells was determined by using Cell Counting Kit-8(CCK-8).Reverse Transcription-Polymerase Chain Reaction(RT-PCR) was used to detect the effect of Res on the expression of NF-κB P65 mRNA in A549 cells.The cell cycle and apoptosis of A549 cells were analyzed by flow cytometry.The NF-κB P65 protein expression in A549 cells was detected by Western blotting.Results Res can inhibit the proliferation of human lung adenocarcinoma A549 cells by down-regulating NF-κB P65,prevent A549 cell cycle,promote cell apoptosis and inhibit the expression of protein.Conclusion Res can significantly inhibit the proliferation of human lung adenocarcinoma A549 cells by down-regulating NF-κB P65.

关 键 词：A549细胞 白藜芦醇 NF-κB P65 增殖 

分 类 号：R734.2[医药卫生—肿瘤]