香椿种子人工老化过程中生理生化的变化  

Physiological and Biochemical Changes of Toona sinensis Seeds During Artificial Aging

作　　者：方娇阳 祝燕 王彩云 叶可可 高卫东[1] 张子晗 闫晶晶 李庆梅[1] FANG Jiao-yang;ZHU Yan;WANG Cai-yun;YE Ke-ke;GAO Wei-dong;ZHANG Zi-han;YAN Jing-jing;LI Qing-mei(Research Institute of Forestry,Chinese Academy of Forestry,Key Laboratory of Tree Breeding and Cultivation,National Forestry and Grassland Administration,State Key Laboratory of Tree Genetics and Breeding,Beijing 100091,China)

机构地区：[1]中国林业科学研究院林业研究所,国家林业和草原局林木培育重点实验室,林木遗传育种国家重点实验室,北京100091

出　　处：《林业科学研究》2020年第6期163-169,共7页Forest Research

基　　金：中国林业科学研究院林业研究所林木培育重点实验室专项资金(ZDRIF201701)。

摘　　要：[目的]通过测定不同老化程度香椿种子的发芽指标、幼苗形态指标和生理生化指标,探究香椿种子人工老化过程中种子活力及其生理生化的变化,为香椿种子老化机制及种子活力修复研究奠定基础。[方法]采用高温高湿(40℃、相对湿度95%)的人工老化方法对供试种子处理1、2、3、4、5、6、7、8 d,测定各处理种子发芽指标、幼苗形态指标、相对电导率、MDA含量和抗氧化酶活性等指标。[结果]香椿种子经老化处理后,种子活力显著下降,相对电导率和MDA含量显著上升。在种子老化过程中,发芽率、发芽指数和活力指数逐渐降低,相对电导率和MDA含量总体呈上升趋势,SOD活性和POD活性表现为先升高后降低的趋势,且SOD对于香椿种子的老化更敏感。相关分析表明:相对电导率、MDA含量与种子发芽指标呈极显著负相关,POD活性与种子发芽指标呈极显著正相关,SOD活性与发芽率呈显著负相关,与发芽指数和活力指数呈极显著负相关。[结论]膜脂过氧化作用和抗氧化酶系统功能失调是香椿种子老化的主要原因,相对电导率、MDA含量和POD活性可用于衡量香椿种子的活力水平。[Objective]To investigate the seed vigor and physiological and biochemical changes of Toona sinensis seeds during artificial aging by measuring the germination indexes,seedling morphological indexes,physiological and biochemical indexes of T.sinensis seeds at different aging degrees.[Method]T.sinensis seeds were treated with artificial aging at high temperature(40℃)and high humidity(RH 95%)for 1 to 8 days respectively.The seed germination indexes,seedling morphological indexes,relative electrical conductivity,MDA content,and antioxidant enzyme activity were measured after treatment.[Result]After the aging treatment,the seed vigor of T.sinensis decreased significantly,the relative electrical conductivity and MDA content increased significantly.During the aging process of seeds,the germination rate,germination index,and vigor index gradually decreased,the relative electrical conductivity and MDA content showed an increasing tendency,the activity of SOD and POD went up at initial and then went down along the seeds aging,and SOD was more sensitive to the aging of T.sinensis seeds.The correlation analysis indicated the relative electrical conductivity and MDA content had extremely and significantly negative correlation with seed germination indexes,while the POD activity showed a extremely and significantly positive correlation with seed germination indexes,the SOD activity was significantly and negatively correlated with germination rate,and extremely and significantly positive correlated with germination index and vigor index.[Conclusion]The peroxidation of membrane lipids and the dysfunction of the antioxidant enzyme system are the main reasons causing the aging of T.sinensis seeds.The relative electrical conductivity,MDA content and POD activity can be used to measure the vigor level of T.sinensis seeds.

关 键 词：香椿 人工老化 种子活力 抗氧化酶 

分 类 号：S644.4[农业科学—蔬菜学]