乳腺癌组织成分变化的拉曼光谱学研究  

Study on Changes of Breast Cancer Tissue Composition by Raman Spectroscopy

作　　者：马丹英 符娟娟 唐金兰 包一麟 尚林伟 王潇[1] 尹建华[1] MA Dan-Ying;FU Juan-Juan;TANG Jin-Lan;BAO Yi-Lin;SHANG Lin-Wei;WANG Xiao;YIN Jian-Hua(Department of Biomedical Engineering,Nanjing University of Aeronautics and Astronautics,Nanjing 210016,China)

机构地区：[1]南京航空航天大学生物医学工程系,南京210016

出　　处：《分析化学》2021年第1期144-150,共7页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.61378087);江苏省六大人才高峰计划项目(No.SWYY-034)。

摘　　要：拉曼光谱学技术可在分子层次上获取组织病变的微观信息。本研究采用显微拉曼光谱系统对20位患者的健康与癌变乳腺组织进行原位拉曼光谱探测,研究组织中生化成分的变化。对健康与癌变乳腺组织的平均拉曼光谱进行对比分析发现,相比于健康组织,癌变组织的光谱强度明显降低。比较两类组织的蛋白质成分与脂质成分的拉曼强度比值发现,癌变组织中的脂质含量减少,蛋白质含量增加。同时,在组织癌变过程中,饱和脂肪酸向不饱和脂肪酸转化,并且多不饱和脂肪酸的含量增加,单不饱和脂肪酸的含量减少。本研究表征了乳腺组织癌变过程中更多生化成分的含量变化,尤其是脂肪酸成分含量的变化,对乳腺癌变的生化机理研究具有重要意义,并有助于乳腺癌的早期监测及光谱学诊断。Raman spectroscopic technique is powerful to obtain microscopic information of tissue lesions at molecular level.In this study,Raman microspectroscopy system was used to collect in situ Raman spectra of the healthy breast and breast cancer tissues of 20 patients to investigate the biochemical composition changes.By comparing the average Raman spectra of healthy and cancerous breast tissues,it was clearly shown that the spectral intensity of cancerous tissues was significantly lower than that of healthy breast tissues.As comparing Raman intensity ratios of the protein and lipid components between the two types of tissues,it was found that the lipid content decreased and the protein content increased in cancerous tissues.In the meantime,saturated fatty acids were converted into unsaturated fatty acids in the process of tissue canceration.And it was further found that polyunsaturated fatty acids increased in content and monounsaturated fatty acids decreased in content after tissue canceration.This study revealed many biochemical component changes in the canceration process of breast tissue,especially the changes in fatty acid content,which was of great significance for the study of the biochemical mechanism of breast cancer,and was helpful for early monitoring and spectroscopic diagnosis of breast cancer.

关 键 词：拉曼光谱 乳腺癌 脂质 蛋白质 脂肪酸 

分 类 号：R737.9[医药卫生—肿瘤]