α-淀粉酶对猪粪厌氧发酵产甲烷的影响及动力学分析  

Effect ofα-Amylase on Methane Production of Pig Manure Anaerobic Fermentation and Its Kinetic Analysis

作　　者：陈彪[1] 黄婧[1] 肖艳春[1,2] CHEN Biao;HUANG Jing;XIAO Yan-chun(Institute of Agricultural Engineering Technology, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China;Fujian Provincial Key Lab of Coastal Basin Environment, Fujian Polytechnic Normal University, Fuqing 350300, China)

机构地区：[1]福建省农业科学院农业工程技术研究所,福建福州350003 [2]福建技术师范学院近海流域环境测控治理福建省高校重点实验室,福建福清350300

出　　处：《中国沼气》2020年第6期37-43,共7页China Biogas

基　　金：福建省科技厅省属公益类科研项目(2014R1015-13,2018R1014-2);近海流域环境测控治理福建省高校重点实验室开放基金(S1-KF1903);福建省自然科学基金资助项目(2020J011374)。

摘　　要：为有效提高厌氧发酵底物的生物产甲烷效率(BDA),探析外源水解酶对厌氧发酵产甲烷过程的影响效应,该研究以猪粪为发酵底物,添加外源α-淀粉酶,采用2种模型拟合厌氧发酵过程。研究显示:在适当范围内添加α-淀粉酶(添加量≤30 mg·g-1)对猪粪厌氧发酵产甲烷具有促进作用,α-淀粉酶的添加量与甲烷产率增加呈正相关性。α-淀粉酶添加量为30 mg·g-1的试验组对甲烷产率的促进作用最为显著,其日产气峰值,甲烷产率,BDA,最大反应速率分别为13829 mL,384.9 mL·g-1VS,69.69%,32.63 mL·g-1d-1,与对照组比分别提高了18.1%,22.1%,22.1%,24.6%,厌氧发酵过程符合Gompertz模型(R2=0.9995)。In order to effectively improve the biodegradability(BDA)of fermentation substrate,and investigate the effect of exogenous hydrolase on methane production of anaerobic fermentation,pig manure was used as the fermentation substrate in this study,and exogenousα-amylase were added and the anaerobic fermentation process was fitted by two kinds of kinetic models.The results showed that the addition ofα-amylase within an appropriate range(≤30 mg·g-1)could promote the methane production of pig manure anaerobic fermentation,and the addition ofα-amylase was positively correlated with the increase of methane production rate.The test group withα-amylase addition of 30 mg·g-1 had the most significant promoting effect on methane production rate.The daily gas peak value,methane production rate,BDA and maximum reaction rate were 13829 mL,384.9 mL·gVS-1,69.69%and 32.63 mL·g-1d-1,respectively,which were 18.1%,22.1%,22.1%and 24.6%higher than those of the control group.The anaerobic fermentation process was in accordance with the Gompertz model(R2=0.999,5).

关 键 词：猪粪 厌氧发酵 Α-淀粉酶 甲烷产率 Gompertz模型 

分 类 号：S216.4[农业科学—农业机械化工程] X713[农业科学—农业工程]