基于PPIA序列检测结直肠癌患者血液游离DNA的临床意义  

PPIA sequence-based detection and clinical significance of serum free DNA in patients with colorectal cancer

作　　者：梁鸿斌[1] 周婉瑜 冯伟龙 曾祥胜[1] 陈新彬 吴锦顺[1] 祝琰[1] LIANG Hong-bin;ZHOU Wan-yu;FENG Wei-long;ZENG Xiang-sheng;CHEN Xin-bin;WU Jin-shun;ZHU Ya(Zhuhai International Travel Healthcare Center,Zhuhai 519000,China;Fifth Affiliated Hospital of Zunyi Medical University,Zhuhai 519000,China)

机构地区：[1]珠海国际旅行卫生保健中心,广东珠海519100 [2]遵义医科大学第五附属<珠海>医院,广东珠海519100

出　　处：《广东医科大学学报》2020年第6期686-688,共3页Journal of Guangdong Medical University

基　　金：珠海市科技计划项目(No.20181117E030011)。

摘　　要：目的建立基于肽基脯氨酰异构酶A(PPIA)序列检测结直肠癌(CRC)患者血液游离DNA(cf-DNA)方法并探讨其临床意义。方法设计特异性引物并利用荧光定量PCR技术建立血液游离DNA检测方法,定量检测结直肠癌患者(A组)、结直肠息肉患者(B组)和健康对照者(C组)各30例的cf-DNA水平;比较肿瘤患者不同TNM分期cf-DNA水平。结果PPIA116扩增曲线:y=-3.936x+11.415,R2=0.995,扩增效率79.5%。PPIA熔解温度为80.00℃,批内CV 0.83%~0.87%,重复性良好。cf-DNA水平:A组最高,B组次之,C组最低(P<0.01)。不同TNM分期患者cf-DNA水平差异具有统计学意义(P<0.01或0.05)。结论基于PPIA序列定量检测cf-DNA具有可行性,监测cf-DNA有助于CRC诊断和分期。Objective To establish a peptide prolyl isomerase A(PPIA)sequence-based method for detection of cell-free DNA(cf-DNA)and explore its clinical significance in patients with colorectal cancer(CRC).Methods cf-DNA levels in 30 patients with CRC(group A),30 with colorectal polyps(group B)and 30 healthy controls(group C)were determined by fluorescence quantitative PCR using specific primers,and then compared among different TNM stages.Results Amplification curve of PPIA 116 was y=-3.936 x+11.415,R2=0.995,and amplification efficiency was 79.5%.Melting temperature of PPIA was 80.00°C,and CV per batch was 0.83%-0.87%,with good repeatability.cf-DNA levels were highest in group A,moderate in group B,and lowest in group C(P<0.01).cf-DNA contents were significantly different among different TNM stages(P<0.01 or 0.05).Conclusion PIA sequence-based detection of cf-DNA is feasible.cf-DNA levels are helpful for diagnosis and staging of CRC.

关 键 词：PPIA序列 结直肠癌 游离DNA 

分 类 号：R735.3[医药卫生—肿瘤]