miR-205靶向E2F1对肾上腺皮质癌SW-13细胞增殖影响  

Effects of miR-205 targeting E2F1 on proliferation of adrenocortical carcinoma SW-13 cells

作　　者：赵兴亮 张帆 刘佳 ZHAO Xing-liang;ZHANG Fan;LIU Jia(Department of Urology,Benxi Central Hospital,Benxi 117000;Laboratory Animal Center,China Medical University,Shenvana,110122,China)

机构地区：[1]本溪市中心医院泌尿外科,辽宁本溪117000 [2]中国医科大学实验动物部,辽宁沈阳110122

出　　处：《解剖科学进展》2020年第6期677-680,共4页Progress of Anatomical Sciences

基　　金：辽宁省自然科学基金(2015020518)。

摘　　要：目的探讨miR-205对肾上腺皮质癌SW-13细胞增殖的影响及机制。方法采用miR-205模拟物转染肾上腺皮质癌SW-13细胞,设置空白组(未进行转染)、miR-205阴性对照组(转染无义序列)、miR-205模拟物转染组(转染miR-205模拟物),采用CCK-8法检测各组SW-13细胞的增殖,实时定量PCR检测miR-205和E2F1表达,Western blot检测SW-13细胞E2F1蛋白表达,应用生物信息学网站预测E2F1是否为miR-205潜在的靶基因,应用双荧光素酶报告基因实验验证。结果 miR-205过表达抑制SW-13细胞增殖,下调SW-13细胞中E2F1mRNA和蛋白表达水平,生物信息学分析结果显示,E2F1是miR-205的靶基因之一,双荧光素酶实验证实E2F1为miR-205的靶基因。结论 miR-205通过靶向调控E2F1抑制肾上腺皮质癌SW-13细胞的增殖。Objective To investigate the effect and mechanism of miR-205 on the proliferation of adrenocortical carcinoma SW-13 cells. Methods SW-13 cells were divided into blank control group(not transfected), miR-205 negative control group(transfected unrelated sequence), miR-205 mimics transfection group(transfected miR-205 mimics). The proliferation of SW-13 cells was detected by CCK-8, the expression of mir-205 and E2 F1 was detected by real-time quantitative PCR, and the expression of E2 F1 protein was detected by Western blot. Bioinformatics and Dual luciferase reporter gene experiment were used to verify that E2 F1 is a potential target gene of miR-205. Results The overexpression of miR-205 downregulated the expression level of E2 F1, and inhibited the proliferation of SW-13 cells. Bioinformatics analysis and dual luciferase reporter gene confirmed that E2 F1 was the target gene of miR-205. Conclusion miR-205 inhibits the proliferation of adrenocortical carcinoma SW-13 cells via targeting E2 F1.

关 键 词：miR-205 E2F1 肾上腺皮质癌SW-13细胞 增殖 

分 类 号：R736.6[医药卫生—肿瘤]