双氢青蒿素抑制TGF-β1诱导的子宫内膜上皮细胞转分化的作用  

Inhibition of dihydroartemisinin on TGF-β1 induced transdifferentiation of endometrial epithelial cells

作　　者：宋玉杰 单铁英[2] 马景红[3] 栗志英[3] 张文博[1] 张巍 李玲 任海霞[3] SONG Yu-jie;SHAN Tie-ying;MA Jing-hong;LI Zhi-ying;ZHANG Wen-bo;ZHANG Wei;LI Ling;REN Hai-ria(Department of Gynecology,Handan Central Hospital,Handan Hebei,056002,China;Medical College,Hebei Engineering University,Handan Hebei,056002,China;Affiliated Hospital,Hebei Engineering University,Handan Hebei,056002,China)

机构地区：[1]邯郸市中心医院妇科,河北邯郸056002 [2]河北工程大学医学院,河北邯郸056002 [3]河北工程大学附属医院,河北邯郸056002

出　　处：《职业与健康》2020年第19期2625-2628,共4页Occupation and Health

摘　　要：目的研究双氢青蒿素(DHA)对转化生长因子-β1(TGF-β1)作用的子宫壁的内膜层中上皮柱状细胞分裂和转分化的影响。方法于2019年6月1日—12月30日,分离正常生育期妇女的子宫壁内膜层中的上皮柱状细胞并调制其浓度为4×10^4个/mL,以200μL/孔的量滴入96孔的细胞培养板中,孵育24 h后,将其在96孔板中划分为4组,各组分别安排6个重复的孔。正常组:常规培养;TGF-β1组:常规培养液中加入TGF-β1;DHA组:常规培养液中加入DHA;TGF-β1+DHA组:常规培养液中加入TGF-β1和DHA。观察各组细胞的分裂增殖情况;ELISA分析培养细胞的上清液中Ⅰ型胶原及纤粘连蛋白的水平;免疫印迹法评估胞质里α-平滑肌肌动蛋白和E-钙粘蛋白的值。结果正常组与DHA组相比较,差异无统计学意义(P>0.05);TGF-β1组与正常组相比,其上皮柱状细胞分裂的数量增多(0.1274±0.0031),上清液里的I型胶原(0.7322±0.0069)及纤黏连蛋白(0.6820±0.0089)的水平升高,细胞质里E-钙粘蛋白值(0.448±0.054)降低,而α-平滑肌肌动蛋白值(0.7220±0.0240)升高,差异有统计学意义(均P<0.05)。TGF-β1+DHA组和TGF-β1组相比,其上皮柱状细胞分裂的数量减少(0.1169±0.0012),上清液里的Ⅰ型胶原(0.6535±0.0101)及纤黏连蛋白(0.5538±0.0100)的水平降低,细胞质里E-钙粘蛋白值(0.6390±0.0330)升高,而α-平滑肌肌动蛋白值(0.4020±0.0260)降低,差异均有统计学意义(均P<0.05)。结论双氢青蒿素能拮抗TGF-β1作用的子宫壁内膜层中的上皮柱状细胞分裂、分化为肌成纤维细胞。Objective To study the effect of dihydroartemisinin(DHA)on the division and transdifferentiation of endometrial columnar epithelial cells incubated with transforming growth factor-β1(TGF-β1).Methods From June 1 to December 30,2019,the epithelial columnar cells were isolated from the endometrium of normal women and the concentration was adjusted to 4×10^4 cells/mL,and the cells were dropped into 96 well cell culture plate,200μL/well.After incubation for 24 h,the cells were divided into four groups in 96 well plate,and six repeated wells in each group.Normal group was given routine culture,TGF-β1 group was given normal medium with TGF-β1,DHA group was given normal medium with DHA,and TGF-β1+DHA group was given normal medium with TGF-β1 and DHA.Cell division and proliferation in each group were observed.The levels of collagen typeⅠand fibronectin in the supernatant were analyzed by ELISA.Western blotting was used to evaluate the values ofα-smooth muscle actin and E-cadherin in the cytoplasm.Results The difference was not statistically significant between normal group and DHA group(P>0.05).Compared with normal group,the number of columnar epithelial cell division in TGF-β1 group(0.1274±0.0031)increased,the levels of collagen type(0.7322±0.0069)and fibronectin(0.6820±0.0089)in the supernatant increased,the E-cadherin value in the cytoplasm(0.448±0.054)decreased,while the value ofα-smooth muscle actin(0.7220±0.0240)increased,and the differences were statistically significant(all P<0.05).Compared with TGF-β1 group,the number of columnar epithelial cell division in TGF-β1+DHA group(0.1169±0.0012)decreased,the levels of collagen type(0.6535±0.0101)and fibronectin(0.5538±0.0100)in the supernatant decreased,the E-cadherin value in the cytoplasm(0.6390±0.0330)increased,while the value ofα-smooth muscle actin(0.4020±0.0260)decreased,and the differences were statistically significant(all P<0.05).Conclusion Dihydroartemisinin could antagonize the division and differentiation of epithelial columnar cell

关 键 词：子宫内膜柱状上皮细胞 TGF-Β1 I型胶原 Α-平滑肌肌动蛋白 E-钙粘蛋白 

分 类 号：R394[医药卫生—医学遗传学]