AtHAP5A regulates iron translocation in iron-deficient Arabidopsis thaliana  

作　　者：Xiao Fang Zhu Qi Wu Yu Ting Meng Ye Tao Ren Fang Shen 

机构地区：[1]State Key Laboratory of Soil and Sustainable Agriculture,Institute of Soil Science,Chinese Academy of Sciences,Nanjing,210008,China [2]University of Chinese Academy of Sciences,Beijing,100049,China

出　　处：《Journal of Integrative Plant Biology》2020年第12期1910-1924,共15页植物学报（英文版）

基　　金：We thank Professor Zhulong Chan(Wuhan Botanical Garden,Chinese Academy of Sciences)for the seeds of the hap5a mutant and the HAP5A-OX;Professor Hongqing Lin(Institute of Genetics and Developmental Biology,Chinese Academy of Sciences)for the seeds of the fit1 mutant and the transgenic lines of OX29/38 and OX29/39;and Professor Chongwei Jin(College of Environmental and Resource Sciences,Zhejiang University)for the seedsof the pye-1mutant.This work was supported by the Youth lnnovation Promotion Association of CAS(2015250);the National Key Basic Research Program of China(2014CB441000);the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB15030302 and XDB15030202).

摘　　要：Iron(Fe)deficient plants employ multiple strategies to increase root uptake and root-to-shoot translocation of Fe.The identification of genes that are responsible for these processes,and a comprehensive understanding of the regulatory effects of transcriptional networks on their expression,including transcription factors(TFs),is underway in Arabidopsis thaliana.Here,we show that a Histone-or heme-associated proteins(HAP)transcription factor(TF),HAP5A,is necessary for the response to Fe deficiency in Arabidopsis.Its ex-pression was induced under Fe deficiency,and the lack of HAP5A significantly decreased Fe translocation from the root to the shoot,resulting in substantial chlorosis of the newly expanded leaves,compared with the wild-type(WT,Col-0).Further analysis found that the expression of a gene encoding nicotianamine(NA)synthase(NAS1)was dramatically decreased in the hap5a mutant,regardless of the Fe status.Yeast-one-hybrid and ChIP analyses suggested that HAP5A directly binds to the promoter region of NAS1.Moreover,overexpression of NAS1 could rescue the chlorosis phenotype of hap5a in Fe deficient conditions.In summary,a novel pathway was elucidated,showing that NAS1-dependent translocation of Fe from the root to the shoot is controlled by HAP5A in Fe-deficient Arabidopsis thaliana.

关 键 词：ARABIDOPSIS TRANSLOCATION expanded 

分 类 号：Q943.2[生物学—植物学]