基于RNA测序研究CUEDC1基因对急性单核细胞白血病THP-1细胞基因表达谱的影响  

Effect of CUEDC1 gene on the gene expression profile of acute monocytic leukemia THP-1 cells based on RNA sequencing

作　　者：柴星星[1] 杜晓鹏 孟凡静[1] 庄万传[1] Chai Xingxing;Du Xiaopeng;Meng Fanjing;Zhuang Wanchuan(Department of Hematology,Lianyungang Hospital Affiliated to Bengbu Medical College,Lianyungang 222023,China;Postgraduate School,Bengbu Medical College,Bengbu 233030,China)

机构地区：[1]蚌埠医学院附属连云港医院血液内科,江苏连云港222023 [2]蚌埠医学院研究生院,安徽蚌埠233030

出　　处：《白血病．淋巴瘤》2020年第11期648-654,共7页Journal of Leukemia & Lymphoma

基　　金：江苏省卫生计划生育委员会"科教强卫工程"医学青年人才项目(QNRC2016495);连云港市第五期"521高层次人才培养工程"科研资助项目。

摘　　要：目的探讨CUEDC1基因对急性单核细胞白血病THP-1细胞基因表达谱的影响。方法构建CUEDC1基因干扰的THP-1细胞差异表达基因库。基于RNA测序技术比较CUEDC1基因干扰的THP-1细胞与阴性对照THP-1细胞基因表达谱的差异性表达。采用实时荧光定量聚合酶链反应验证差异表达的部分基因。将表达上调和下调的基因分别导入DAVID软件,进行基因本体(GO)功能富集分析。结果成功构建CUEDC1基因干扰的THP-1细胞差异表达基因库。CUEDC1干扰组与阴性对照组间共检测到161个差异表达基因(P<0.05),其中显著上调基因85个,显著下调基因76个;与细胞增殖相关的基因9个,与细胞凋亡相关的基因10个,与p53基因有关的基因2个,与转录调控有关的基因3个,与泛素化有关的基因8个;其中SMAD5、SG15、CBLL1、FANCF等基因与急性白血病的发生发展密切相关。结论CUEDC1基因可能影响SMAD5、SG15、CBLL1、FANCF等基因的表达,进而参与了急性单核细胞白血病的发生发展。Objective To investigate the effect of CUEDC1 gene on the acute monocytic leukemia THP-1 cells gene expression profile.Methods The differential expression gene bank of THP-1 cells with CUEDC1 gene interference was constructed.The differential gene expression of THP-1 cells in CUEDC1 interference group and the negative control group was compared based on RNA sequencing technology.The part of the differentially expressed genes were verified by using real-time polymerase chain reaction(PCR),and the up-regulated and down-regulated genes were respectively imported into DAVID software for gene ontology(GO)function enrichment analysis.Results The differentially expressed gene bank of THP-1 cells interfered by CUEDC1 gene was successfully constructed.A total of 161 differentially expressed genes were detected in CUEDC1 interference group and the negative control group(P<0.05),including 85 up-regulated genes and 76 down-regulated genes.There were 9 genes related to cell proliferation and 10 genes related to apoptosis,2 genes related to p53 gene and 3 genes related to transcriptional regulation,8 genes related to ubiquitin,among which SMAD5,SG15,CBLL1,FANCF and other genes were closely related to the occurrence and development of acute leukemia.Conclusion CUEDC1 gene participates in the occurrence and development of acute monocytic leukemia by influencing the expressions of SMAD5,SG15,CBLL1,FANCF and other genes.

关 键 词：白血病 单核细胞 急性 序列分析 RNA 基因表达谱 基因 CUEDC1 

分 类 号：R733.71[医药卫生—肿瘤]