高效液相色谱法同时测定蔷薇科木本植物油中的苯甲醇和苯甲醛  

Simultaneous Determination of Benzyl Alcohol and Benzaldehyde in Woody Oil of Rosaceae by High Performance Liquid Chromatography

作　　者：朱琳 张蕊 郝希成 王松山 王松雪 郭宝元 薛雅琳 张东 刘鑫辉 郭咪咪 Zhu Lin;Zhang Rui;Hao Xichen;Wang Songshan;Wang Songxue;Guo Baoyuan;Xue Yalin;Zhang Dong;Liu Xinhui;Guo Mimi(Academy of National Food and Strategic Reserves Administration,Beijing 100037;Lüliang University,Lüliang 033000)

机构地区：[1]国家粮食和物资储备局科学研究院,北京100037 [2]吕梁学院,吕梁033000

出　　处：《中国粮油学报》2020年第12期170-176,共7页Journal of the Chinese Cereals and Oils Association

基　　金：国家粮食与物资储备局粮食行业标准(HB1916)。

摘　　要：建立了一种同时测定蔷薇科木本植物油中苯甲醇和苯甲醛的高效色谱法方法。样品经甲醇提取后,采用具有紫外检测器的液相色谱仪测定,色谱条件:Agilent SB-C18柱(250 mm×4.6 mm,5μm)色谱柱,甲醇∶0.02%磷酸水=30∶70为流动相,柱温为30℃,流速为1.0 mL/min,苯甲醇检测波长为210 nm,苯甲醛检测波长为250 nm。结果表明,苯甲醇加标平均回收率为91.8%~93.7%,精密度为0.6%~2.0%;苯甲醛加标平均回率为96.5%~97.7%,精密度为0.2%~2.9%;苯甲醇的检出限为0.41 mg/kg,定量限为1.23 mg/kg;苯甲醛的检出限为0.33 mg/kg,定量限为0.98 mg/kg。本方法不仅回收率高,重复性好,而且灵敏度高,可用于测定杏仁油、长柄扁桃油等蔷薇科木本植物油中的苯甲醇和苯甲醛含量,为科学评价蔷薇科木本油脂产品的风味提供了技术支撑。A HPLC method for the simultaneous determination of benzyl alcohol and benzaldehyde in apricot kernel oil and amygdalus pedunculata pall oil was established.The sample was extracted by methanol,then analyzed in a HPLC instrument coupled with UV detector.Chromatographic condition:an Agilent SB-C18 column(250 mm×4.6 mm,5μm)at 30℃.Methanol:0.02%phosphoric acid water(30%)was used as mobile phase at a constant flow rate of 1.0 mL/min.The detection wavelength was set at 210 nm for benzyl alcohol and 250 nm for benzaldehyde.The results indicated that the average recovery rate of benzyl alcohol and benzaldehyde were 91.8%~93.7%(relative standard deviations,0.6%~2.0%)and 96.5%~97.7%(relative standard deviations,0.2%~2.9%),individually.The detection limit and quantitative limit of benzyl alcohol were 0.41 mg/kg and 1.23 mg/kg,respectively.The detection limit and quantitative limit of benzaldehyde were 0.33 mg/kg and 0.98 mg/kg,respectively.The method is not only highly recoverable and reproducible,but also highly sensitive,and can be used to determine the content of benzyl alcohol and benzaldehyde in rosaceae woody oils such as apricot kernel oil and amygdalus pedunculata pall oil.Based on the above reasons,this method provides technical support for the scientific evaluation of the flavor of rosaceae woody oil products.

关 键 词：杏仁油 长柄扁桃油 苯甲醇 苯甲醛 高效液相色谱 

分 类 号：TS227[轻工技术与工程—粮食、油脂及植物蛋白工程]