Traditional Chinese Medicines alleviated myocardial ischemia by regulating Qi and promoting blood circulation  

作　　者：Wang Chen Wang Xiaoreng Tao Tianqi Song Dandan Wang Jianli Wang You Liu Xiuhua Wu Xudong 

机构地区：[1]Institute of Basic Theory of Traditional Chinese Medicine,China Academy of Chinese Medical Science,Beijing 100700,China [2]Out-patient Department,Chinese PLA General Hospital,Beijing 100853,China [3]Department of Pathophysiology,Chinese PLA General Hospital,Beijing 100853,China

出　　处：《Journal of Traditional Chinese Medicine》2020年第6期974-982,共9页中医杂志（英文版）

基　　金：Supported by National key basic research and development plan(973 Program)(No.2015CB554405,2015CB554402)。

摘　　要：OBJECTIVE:To investigate the efficacy of Chinese medicines on Qi stagnation and blood stasis in rats with myocardial ischemia.METHODS:Fifty male Wistar rats were randomly divided into five groups(n=10)as follows:(a)sham operation(Sham),(b)myocardial ischemia(Model),(c)treatment that regulates Qi(Qi),(d)treatment that promotes blood circulation(Blood),(e)treatment that both regulates Qi and promotes blood circulation(QB).The rat model was established via activities restriction for 6 h followed by tail clamp stimulation for 5 mins every day for 7 d and occlusion left coronary anterior descending artery.Afterwards rats were treated with medicines that regulate Qi and/or promote blood circulation via gavage for 14 d.Behavioral parameters were evaluated using open field and elevated plus-maze tests.The tongue color and sublingual vein were visually examined.Blood flow perfusion of tongue and auricle were detected using PIMⅡ.The mesenteric microcirculation was examined via capillaroscopy,and hemodynamics was assessed using a polygraph system.Serum homocysteine(Hcy),creatine kinase isoenzyme(CKMB)levels and endothelin-1(ET-1)were measured.Hematoxylin and eosin staining and transmission electron microscopy were employed to detect the myocardial morphology and ultrastructure,respectively.RESULTS:Compared with findings in Sham group,rats in model group had coarse hair,dark mucosa of the lips and claw,low activity,and increased anxiety.Compared with findings in Model group,rats in the three treatment groups exhibited a lighter tongue color without an extended and varicose sublingual vein.There were significant increases of auricle blood flow perfusion in the Qi group and tongue bottom blood flow perfusion in the QB group.Compared with findings in Model rats,rats in Blood group exhibited improved mesenteric microcirculation associated with increased mesenteric blood flow and a larger arteriole diameter.Moreover,compared with findings in Model rats,Qi and QB rats exhibited increased left ventricular±dp/dtmax,decreased seru

关 键 词：Myocardial ischemia HOMOCYSTEINE ENDOTHELIN-1 Creatine kinase ISOENZYMES Qi-stagnation and blood-stasis Regulating Qi and promoting blood circulation 

分 类 号：R285.5[医药卫生—中药学]