Maxingxiongting mixture attenuates hypoxia pulmonary arterial hypertension to improve right ventricular hypertrophy by inhibiting the rho-kinase signaling pathway  

作　　者：An Xing Li Songtao Weng Xiangwen Wang Xian Wu Hao Zhang Xinyue Gao Jian Yang Renxu Peng Bo 

机构地区：[1]Respiration Department,Hospital of Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China [2]Oncology Department,Hospital of Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China [3]Critical Care Medicine Department,Hospital of Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China [4]Endocrine Department,Hospital of Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China [5]Chengdu University of Traditional Chinese Medicine,Hospital of Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China

出　　处：《Journal of Traditional Chinese Medicine》2020年第6期992-998,共7页中医杂志（英文版）

基　　金：Supported by State Administration of Traditional Chinese Medicine"Hundred Million"Talent Project of Inheritance and Innovation of Traditional Chinese Medicine National Old Chinese Medicine Experts Academic Experience Inheritance Project(Issued by the Ministry of Education of Traditional Chinese Medicine 2017 No.29);Technological Innovation Project"Baitang"Physical Examination and Analysis of the Significance of Prescription;Sichuang Provincial Science and Technology Department(No.2018RZ0126)。

摘　　要：OBJECTIVE:To explore the mechanism of Maxingxiongting mixture(MXXTM)on pulmonary hypertension in a rat model established by intraperitoneal injection of monocrotaline solution,smoking and forced swimming.METHODS:A total of 30 male Sprague-Dawley rats were randomly divided into five groups:control group,model group,high-dose of MXXTM group(HM),low-dose of MXXTM group(LM),and fasudil group.The mean pulmonary artery pressure(m PAP)was measured by using a miniature catheter.Lung tissue and right ventricular tissue sections were stained with hematoxylin-eosin.The right ventricle(RV)and left ventricle+septum(LV+S)were weighted.RV/(LV+S)was calculated to reflect the degree of right ventricular hypertrophy.Rho/Rho-kinase signaling pathway key proteins(Rho A,ROCKⅠand ROCKⅡ)in rat right ventricular tissue were measured by Western blot analysis.The levels of serum hypoxia-inducible factor-1α(HIF-1α),vascular endothelial growth factor(VEGF)and the levels of plasma renin activity(PRA),angiotensinⅡ(ANG-Ⅱ),aldosterone(ALD)in rat anticoagulated plasma were all measured by enzyme-linked immunosorbent assay.RESULTS:Compared with the control group,the m PAP and RV/(LV+S)in the model group were significantly increased.Administration of fasudil resulted in a significant decrease of m PAP and RV/(LV+S).In the HM group and LM group,m PAP and RV/(LV+S)were significantly lower than the model group.Compared with the control group,the contents of HIF-1α,VEGF,PRA,ANG-Ⅱand ALD in the model group were significantly increased.The administration of fasudil and high-dose MXXTM significantly reduced the contents of HIF-1α,VEGF,PRA,ANG-II and ALD.Compared with the control group,the expression of Rho A,ROCKⅠand ROCKⅡin the right ventricle of the model group were significantly increased.The administration of fasudil and high-dose MXXTM significantly reduced the expression of Rho A and RockⅡproteins.Our results indicated that high-dose of MXXTM had similar effects on reducing pulmonary artery pressure and improving right ventricul

关 键 词：Pulmonary arterial hypertension Rho A GTP-binding protein FASUDIL Maxingxiongting mixture 

分 类 号：R285.5[医药卫生—中药学]