GAB1通过PI3K通路对甲状腺癌细胞增殖和淋巴管生成的影响  

Effects of GAB1 on proliferation of thyroid cancer cells and lymphangiogenesis through PI3K pathway

作　　者：石向阳[1] 杨海波[1] 韩江南[1] SHI Xiangyang;YANG Haibo;HAN Jiangnan(Department of Otolaryngology Head and Neck Surgery,Ya’an People’s Hospital,Ya'an,Sichuan,625000,China)

机构地区：[1]雅安市人民医院耳鼻咽喉头颈外科,四川雅安625000

出　　处：《中国耳鼻咽喉头颈外科》2020年第11期615-618,共4页Chinese Archives of Otolaryngology-Head and Neck Surgery

基　　金：四川省卫生厅科研课题(110581);国家自然科学基金资助项目(30772410)。

摘　　要：目的探究GAB1通过PI3K通路对甲状腺癌细胞增殖和淋巴管生成的影响。方法将人甲状腺癌细胞系SW579分为对照组、siGAB1组和GAB1组,通过质粒转染沉默和过表达GAB1。检测各组细胞增殖、迁移和侵袭的能力。检测淋巴管生成标志物CD34和LYVE-1以及PI3K/AKT通路水平。结果转染后,siGAB1组的GAB1 mRNA和蛋白分别从3.25±0.24和2.42±0.22降低至0.54±0.05和0.92±0.10(P<0.05),GAB1组的GAB1 mRNA和蛋白升高至10.65±0.92和4.35±0.40(P<0.05)。siGAB1组的细胞增殖、迁移和侵袭能力显著低于对照组(P<0.05),GAB1组的上述指标显著高于对照组(P<0.05)。siGAB1组的CD34、LYVE-1、PI3K和AKT水平显著低于对照组(P<0.05),GAB1组的上述指标显著高于对照组(P<0.05)。结论GAB1可通过促进PI3K通路促进甲状腺癌细胞增殖、迁移、侵袭和淋巴管生成。OBJECTIVE To explore the effect of GAB1 on the proliferation of thyroid cancer(TC)cells and lymphangiogenesis through the PI3K pathway.METHODSThe human TC cell line cells-SW579 were divided into control group,siGAB1 group and GAB1 group.GAB1 was silenced and overexpressed by plasmid transfection.The ability of cell proliferation,migration and invasion of each group was tested.The levels of CD34,LYVE-1 and PI3K/AKT pathways in lymphatic vessels were detected.RESULTSAfter transfection,GAB1 mRNA and protein of siGAB1 group decreased from 3.25±0.24 and 2.42±0.22 to 0.54±0.05 and 0.92±0.10(P<0.05),respectively.GAB1 mRNA and protein in GAB1 group increased to 10.65±0.92 and 4.35±0.40(P<0.05).The cell proliferation,migration and invasion ability of siGAB1 group was significantly lower than that of control group(P<0.05),and the above indexes of GAB1 group were significantly higher than that of control group(P<0.05).The levels of CD34,LYVE-1,PI3K and AKT in the siGAB1 group were significantly lower than those in the control group(P<0.05),and the above indicators in the GAB1 group were significantly higher than those in the control group(P<0.05).CONCLUSION GAB1 can promote TC cell proliferation,migration,invasion and lymphangiogenesis by promoting the PI3K pathway.

关 键 词：甲状腺肿瘤 淋巴管生成 细胞增殖 Grb2相关结合蛋白1 PI3K通路 

分 类 号：R736.1[医药卫生—肿瘤]