有机硅对蛋白激发子AMEP功能活性的影响  

Effect of Organosilicon on Activity of Protein Elicitor AMEP

作　　者：孙轼絮 郭世伦 张斌斌 李惠洋 刘鸿雁 刘权[1] SUN Shixu;GUO Shilun;ZHANG Binbin;LI Huiyang;LIU Hongyan;LIU Quan(Heilongjiang Bayi Agricultural University,Daqing Heilongjiang 163319)

机构地区：[1]黑龙江八一农垦大学,黑龙江大庆163319

出　　处：《现代农业科技》2021年第1期123-125,131,共4页Modern Agricultural Science and Technology

基　　金：黑龙江省大学生创新创业训练计划项目“AMEP蛋白多聚体与功能活性的关系”(201910223048);黑龙江省八一农垦大学试验示范基地项目“AMEP蛋白免疫激活剂在九三大豆的应用”。

摘　　要：为了明确加入表面活性剂是否会改变AMEP蛋白的亲水和疏水分布,以致影响蛋白的功能活性,本文设置了一系列浓度梯度的有机硅对AMEP蛋白进行处理,通过过敏反应试验来检测有机硅对AMEP蛋白功能活性的影响,并通过灰霉菌接病试验检验了含1/7500有机硅的AMEP蛋白制剂对植物抗病性的影响。结果表明:在有机硅浓度低于1/5000时AMEP蛋白的过敏反应活性有明显增强,而浓度高于1/5000时有机硅对AMEP的过敏反应活性则起到抑制作用;喷施了1/7500有机硅与AMEP混合液的烟草叶片能够显著提升对灰霉菌的抗性。本研究结果表明,合理利用有机硅可以增强AMEP蛋白的功能活性,为有机硅与AMEP的共同施用提供了理论依据。In order to determine whether the addition of surfactant will change the hydrophilic and hydrophobic distribution of AMEP protein,thus affecting the functional activity of the protein,this paper set up a series of organosilicon with concentration gradient to treat AMEP protein,and the effect of organosilicon on AMEP protein functional activity were studied through the symptoms of hypersensitive reaction,and the effect of AMEP protein preparation that contained 1/7500 organosilicon on plant disease resistance were examined through the Botrytis cinerea inoculation experiment.The results showed that the activity of AMEP was promoted significantly when the organosilicon concentration was below 1/5000,and it was inhibited when the organosilicon concentration was higher than 1/5000.Furthermore,tobacco leaves sprayed with the mixture of 1/7500 organosilicon and AMEP were significantly improve the resistance to Botrytis cinerea.This results indicated that reasonable utilization of organosilicon could increase the functional activity of AMEP proteins,which could provided a theoretical basis for the joint application of organosilicon and AMEP.

关 键 词：AMEP 蛋白激发子 功能活性 表面活性剂 有机硅 

分 类 号：TQ423[化学工程]