肠道菌群紊乱在大鼠非酒精性脂肪肝中的作用机制  

Mechanism of intestinal flora disturbance in nonalcoholic fatty liver disease in rats

作　　者：耿妍[1] 鲁晓岚[2] 耿燕[3] 史海涛[2] GENG yan;LU Xiaolan;GENG yan;SHI Haitao(Department of Pediatrics,the Second Affiliated Hospital of Xi'an Jiaotong University,Xi'an,Shanxi,China,710000;Department of Gastroenterology,the Second Affiliated Hospital of Xi'an Jiaotong Universi-ty,Xi'an,Shanxi,China,710000;Clinical Laboratory,the Second Affiliated Hospital of Xi'an Jiaotong University,Xi'an,Shanxi,China,710000)

机构地区：[1]西安交通大学第二附属医院小儿内科,陕西西安710000 [2]西安交通大学第二附属医院消化内科,陕西西安710000 [3]西安交通大学第二附属医院检验科,陕西西安710000

出　　处：《分子诊断与治疗杂志》2020年第12期1626-1630,共5页Journal of Molecular Diagnostics and Therapy

基　　金：陕西省科技攻关科研基金[2007-k14-02(15)]。

摘　　要：目的探讨肠道菌群紊乱在大鼠非酒精性脂肪肝(NAFLD)中的作用机制。方法将30只大鼠随机分为2组:对照组(NC组)(普通饲料,n=6)、模型组(高脂饲料,n=24)。模型组经高脂饮食喂养至12周,取肝脏组织,根据肝脏病理分为单纯性肥胖组(SO组)(n=12)与NAFLD组(n=12)。取大鼠血清,生化仪测定谷丙转氨酶(ALT)、血清谷草转氨酶(AST)、甘油三酯(TC)、胆固醇(TG);采用动态比浊法测定血清内毒素(ET)水平;收集肠道粪便采用RT-PCR分析肠道菌群改变;取肝脏及末端回肠行苏木精伊红(HE)染色观察组织病理。结果 SO组及NAFLD组大肠杆菌属计数、TC、TG、ET均显著高于NC组,差异具有统计学意义(P<0.05)。SO组乳酸杆菌、双歧杆菌属计数均显著低于NC组,差异具有统计学意义(P<0.05)。NAFLD组大肠杆菌属计数、ET均显著高于SO组,双歧杆菌属计数显著低于SO组,差异均具有统计学意义(P<0.05)。大肠杆菌属与ET、肝脏脂肪变性程度均呈正相关(P<0.05),乳酸杆菌属、双歧杆菌属数量与ET、肝脏脂肪变程度均呈负相关(P<0.05)。结论单纯性肥胖时肠道菌群紊乱、肠道屏障的损伤可能是促使内毒素升高、进展为NAFLD的始动因素。Objective To explore the promotion of intestinal endotoxemia induced by intestinal flora disturbance on the conversion of simple obesity into NAFLD.Methods Thirty rats were randomly di⁃vided into 2 groups,the control group(normal diet,n=6),and the model group(high fat diet n=24).After model establishment by high⁃fat diet,the model group was divided into simple obesity group(n=12)and NAFLD(n=12)group according to liver pathology.The rat serum was taken,ALT,AST,TG and TC were measured by biochemical analyzer.The serum endotoxin level was determined by kinetic turbidimetric method.Intestinal flora changes were analyzed by RT⁃PCR.Liver and terminal ileum were taken for HE staining to ob⁃serve histopathology.Results Compared with the normal control group,Escherichia coli,TC,TG and ET in the simple obesity group and the NAFLD group were significantly increased,with statistically significant differences(P<0.05),and the number of Lactobacillus and Bifidobacterium were significantly decreased,with statistically significant differences(P<0.05),the counts of bifidobacteria was significantly lower than that in simple obesity group,with statistically significant differences(P<0.05),Escherichia coli was positively corre⁃lated with ET and liver steatosis(P<0.05),and the number of lactobacillus and bifidobacterium were negative⁃ly correlated with ET and liver steatosis(P<0.05).Conclusion The disturbance of intestinal flora and the damage of intestinal barrier in the simple obesity may be the initiating factors which promoting the rise of endo⁃toxin and progress to NAFLD.

关 键 词：肠道菌群 内毒素 非酒精性脂肪肝 单纯性肥胖 

分 类 号：R575.5[医药卫生—消化系统]