一株褐煤内源菌对平庄褐煤的溶解转化  

Biodissolution and Transformation of Pingzhuang Lignite by a Lignite Endophyte

作　　者：成建国 马力通[1,2] 赵文渊 刘云颖 林雨[3] CHENG Jianguo;MA Litong;ZHAO Wenyuan;LIU Yunying;LIN Yu(School of Chemistry and Chemical Engineering, Inner Mongolia University of Science and Technology, 014010 Baotou, China;Inner Mongolia Engineering Research Center of Comprehensive Utilization of Bio-coal Chemical Industry, 014010 Baotou, China;College of Chemistry and Chemical Engineering, Taiyuan University of Technology, 030024 Taiyuan, China)

机构地区：[1]内蒙古科技大学化学与化工学院,内蒙古包头014010 [2]生物煤化工综合利用内蒙古自治区工程研究中心,内蒙古包头014010 [3]太原理工大学化学与化工学院,太原030024

出　　处：《煤炭转化》2021年第1期26-34,共9页Coal Conversion

基　　金：国家自然科学基金资助项目(21766025);内蒙古自治区科技计划项目(201702027,2020GG0158);内蒙古科技大学创新基金项目-优秀青年科学基金项目(2017YQL03);内蒙古科技大学创新基金项目(2019QDL-B20);内蒙古自治区重大基础研究开放课题资助项目(0406091701).

摘　　要：从内蒙古平庄褐煤中分离到一株高效溶解转化褐煤真菌,命名为HM-M5,并对该菌株进行了基因鉴定、产胞外酶特性及溶煤效果研究,利用扫描电子显微镜(SEM)和气相色谱-质谱联用仪(GC-MS)分别对褐煤形貌和溶解转化产物进行了分析。结果表明:菌株HM-M5经基因鉴定为白色拟青霉(Simplicillium lanosoniveum),在液体培养条件下,褐煤可促进菌株HM-M5分泌漆酶(Lac)和木质素过氧化物酶(Lip),且该菌株对酸处理褐煤溶解转化率高达66.2%。SEM分析结果显示,菌丝可以侵入褐煤内部,在胞外过氧化物酶系作用下将褐煤大分子结构溶解转化为小分子物质。GC-MS分析结果表明,经过萃取得到的4种芳香类产物分别为1,6-二甲基-4-(1-甲基乙基)萘、邻苯二甲酸辛二酯、7-异丙基-1,1,4a-三甲基-1,2,3,4,4a,9,10,10a-八氢菲和1-甲基-7-(1-甲基乙基)菲,且这4种物质的相对含量占总萃取物含量的91.4%,这些物质均为价值较高的化工原料中间体。实验结果证明本研究分离到的拟青霉可高效溶解转化褐煤,主要的褐煤转化产物为低相对分子质量的芳香类物质,且产物种类较少,易于分离纯化,更易于工业化应用。A strain termed as HM-M5 was isolated from the Pingzhuang lignite.The characteristics of the extracellular enzyme production and the coal dissolving effect of the strain were studied.The morphology of the lignite and the products of its biotransformation were analyzed by using the SEM and GC-MS.The results show that the strain HM-M5 is identified as Simplicillium lanosoniveum based on the gene identification result.Under the submerged culture,the lignite can promote the production of the laccase and lignin peroxidase through HM-M5,and the dissolution conversion rate of the acid-treated lignite is as high as 66.2%.The SEM results show that the mycelium can invade the interior of the lignite and dissolve the macromolecular structure of the lignite into small-molecular matters through the extracellular peroxidase systems.The GC-MS analysis show that four kinds of aromatic products are mainly obtained,which are 1,6-dimethyl-4-(1-methylethyl)naphthalene;1,2-octylphthalate;7-isopropyl-1,1,4a-trimethyl-1,2,3,4,4a,9,10,10a-octahydrophenanthrene and 1-methyl-7-(1-methylethyl)phenanthrene,respectively.And these four substances,which are high-value chemical raw material intermediates,account for 91.4%of the total extract.The experimental results show that the Simplicillium lanosoniveum isolated in the present study can efficiently dissolve and transform the lignite.The main lignite conversion products are low-molecular-weight aromatic substances,and the products are easy to be separated and purified,beneficial to the future's industrialization.Therefore,it is of great significance to use the strain to dissolve and transform the lignite to prepare high-value chemicals.

关 键 词：褐煤 气相色谱-质谱 白色拟青霉 木质素过氧化物酶 生物转化 高值化学品 

分 类 号：TQ533[化学工程—煤化学工程]