Dihydroartemisinin induces ER stress-dependent apoptosis of Echinococcus protoscoleces in vitro  

作　　者：Rongji Ma Wenjuan Qin Yuanmao Xie Ziwei Han Shuojie Li Yufeng Jiang Hailong Lv 

机构地区：[1]Emergency Department of the First Affiliated Hospital of the Medical College of Shihezi University,Shihezi 832002,China [2]Department of Ultrasound Diagnosis,The First Affiliated Hospital,School of Medicine,Shihezi University,Shihezi 832000,China [3]School of Basic Medicine,Chengdu Medical College,Chengdu 610500,China [4]Hepatological Surgery Department,The Third People’s Hospital of Chengdu,Chengdu 610500,China [5]Shihezi University School of Medicine,Clinical Pathology Department of the First Affiliated Hospital of the Medical College of Shihezi University,Shihezi 832000,China [6]Department of Gastroenterology of the First Affiliated Hospital of Medical College of Shihezi University,Shihezi 832000,China

出　　处：《Acta Biochimica et Biophysica Sinica》2020年第10期1140-1147,共8页生物化学与生物物理学报（英文版）

基　　金：This work was supported by the grants from the National Natu-ral Science Foundation of China(Nos.81360410,81560334,and U1303121).

摘　　要：In this study,we investigated the effect of dihydroartemisinin on Echinococcus protoscoleces and explored the role of endoplasmic reticulum stress in this process.Echinococcus protoscoleces were collected and cultured in RPMI 1640 medium.Changes in the expressions of glucose-regulated protein 78(GRP-78),caspase-12,and C/EBP homologous protein(CHOP)were assessed through confocal immunofluorescence and western blot analysis.Cell viability and morphological changes were observed under a light microscope.The ultrastructure of protoscoleces was observed by scanning electron microscopy and transmission electron microscopy.Caspase-3 activity was detected using an enzyme assay kit.After dihydroartemisinin treatment,the protoscoleces showed loss of viability,and morphological changes including soma contraction,blebs formation,hooks loss,microtrichia destruction,and development of lipid droplets was observed.The levels of caspase-12 and CHOP were increased within 2 days of dihydroartemisinin treatment.However,the levels of GRP-78,caspase-12,and CHOP were decreased in 4 days.Furthermore,caspase-3 activity was increased after treatment with different concentrations of dihydroartemisinin.Dihydroartemisinin can induce apoptosis in protoscoleces via the ER stress-caspase-3 apoptotic pathway in vitro.These results indicate that dihydroartemisinin is a potentially valuable therapeutic agent against echinococcosis.

关 键 词：DIHYDROARTEMISININ oxidative stress ER stress APOPTOSIS 

分 类 号：Q25[生物学—细胞生物学]