Asiat对脑梗死大鼠氧化应激、Fos蛋白及Ki67蛋白水平的影响  

Effects of Asiat on Oxidative Stress,Fos Protein and Ki67 Protein in Rats with Cerebral Infarction

作　　者：齐凡星[1] 胡莹[2] 李静[1] 张静[1] 张欣[1] 高娟[1] QI Fanxing;HU Ying;LI Jing;ZHANG Jing;ZHANG Xin;GAO Juan(Department of Neurology,Baoding No.1 Central Hospital,Baoding 071000,China;Department of Cardiovascular Medicine,Baoding No.1 Central Hospital,Baoding 071000,China)

机构地区：[1]保定市第一中心医院神经内科,保定071000 [2]保定市第一中心医院心血管内科,保定071000

出　　处：《中国细胞生物学学报》2020年第11期1941-1950,共10页Chinese Journal of Cell Biology

基　　金：保定市科技计划(批准号:1951ZF053)资助的课题。

摘　　要：探讨Asiat对脑梗死大鼠氧化应激、Fos蛋白及Ki67蛋白水平的影响。将60只大鼠平均分为假手术组(sham组)、脑梗死大鼠模型组(CI组)和脑梗死大鼠模型加Asiat治疗组(Asiat组)。对三组大鼠的神经行为学、脑积水量、脑梗死体积进行评定;ELISA检测细胞因子浓度;用HE染色观察脑组织形态的改变;TUNEL染色检测神经元细胞凋亡情况;用Western blot检测Fos蛋白和Ki67蛋白的表达情况。结果发现,CI组的NO和MDA浓度比sham组大鼠显著增加(P<0.05),CI组大鼠SOD的活性显著降低(P<0.05);和CI组大鼠相比,Asiat组大鼠的NO和MDA浓度明显下降,SOD的活性显著升高(P<0.05)。CI组大鼠的脑组织积水明显增多,脑梗死体积也显著增加,并且脑组织神经元细胞也出现了大量的凋亡;CI组大鼠细胞质的染色不均匀,细胞核的染色较深,神经元的数量显著降低;Asiat组大鼠的脑组织积水量明显减少,脑梗死的体积显著降低,细胞凋亡数量减少,脑组织形态明显改善。与sham组相比较,CI组大鼠Fos蛋白含量明显增多,Ki67蛋白含量显著减少(P<0.05);Asiat组大鼠Fos蛋白含量比CI组大鼠显著减少,Ki67蛋白含量比CI组大鼠明显增多(P<0.05)。该研究结果表明,Asiat可抑制脑梗死大鼠氧化应激反应,抑制Fos蛋白表达,促进Ki67蛋白表达,进而对脑组织起到保护作用。To explore the effects of Asiat on oxidative stress and the protein expression of Fos and Ki67 in rats with CI(cerebral infarction),sixty rats were randomly assigned into sham group(sham operation group),CI group(CI model group)and CI operation plus Asiat group(Asiat treatment group).Then,the neurobehavioral,hydrocephalus,and CI volume of rats in each group were evaluated.Cytokine concentration was detected by ELISA.Morphology of brain tissues was observed by HE staining.Additionally,neuronal cell apoptosis was detected by TUNEL staining.Fos protein and Ki67 protein expression were detected by Western blot.Results showed that the concentrations of NO and MDA were significantly higher,but the activity of SOD was reduced in the CI group compared with those in the sham group(P<0.05).Compared with rats in the CI group,the concentrations of NO and MDA decreased significantly,and the activity of SOD increased significantly in the Asiat group(P<0.05).In CI group,the amount of hydrocephalus in brain tissues,the volume of CI and the apoptosis of neuronal cells increased significantly compared with sham group.In addition,the cytoplasm was stained unevenly;the nucleus was stained deeper;and the number of neurons was lower in CI group than those in sham group.On the contrary,the hydrocephalus,CI and neuronal cells apoptosis were obviously attenuated,and the morphology of brain tissues was significantly improved in the Asiat group.Compared with the sham group,the expression of Fos protein increased,while the expression of Ki67 protein decreased in the CI group(P<0.05).However,Asiat treatment recovered Fos and Ki67 expressions(P<0.05).In conclusion,this study showed that Asiat could inhibit the oxidative stress response of rats with CI,and the expression of Fos protein,and promote the expression of Ki67 protein,thereby exhibiting its protective effect on brain tissues.

关 键 词：Asiat 脑梗死 氧化应激 FOS蛋白 KI67蛋白 

分 类 号：R743.33[医药卫生—神经病学与精神病学]