背角无齿蚌幼蚌心率对镉暴露胁迫的响应及预警评估  

Response of Heart Rates to Cadmium-Exposed Stress and Early Warning Capacity in Anodonta woodiana Juveniles

作　　者：陈修报[1] 王安琪 郑浩然 刘洪波[1] 杨健[1,2] CHEN Xiubao;WANG Anqi;ZHENG Haoran;LIU Hongbo;YANG Jian(Key Laboratory of Fishery Eco-Environment Assessment and Resource Conservation in Middle and Lower Reaches of the Yangtze River,Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081;Wuxi Fisheries College,Nanjing Agricultural University,Wuxi 214081)

机构地区：[1]中国水产科学研究院淡水渔业研究中心,中国水产科学研究院长江中下游渔业生态环境评价和资源养护重点实验室,无锡214081 [2]南京农业大学无锡渔业学院,无锡214081

出　　处：《渔业科学进展》2021年第1期186-192,共7页Progress in Fishery Sciences

基　　金：中国水产科学研究院基本科研业务费(2019GH10);国家自然科学基金(31502166);江苏省自然科学基金(BK20161144);中国水产科学研究院淡水渔业研究中心基本科研业务费(2019JBFM05)共同资助。

摘　　要：为探究“淡水贝类观察”研究体系专用指示生物“标准化”背角无齿蚌(Anodonta woodiana)幼蚌的心率对镉(Cd)暴露胁迫的响应及预警评估,在“非致死/非损伤”条件下,研究从心跳清晰可见至贝壳不再透明的典型生长阶段的早期幼蚌:EJ1[25日龄,壳长为(1.5±0.1)mm]、EJ2[55日龄,壳长为(10.5±0.6)mm]、EJ3[70日龄,壳长为(20.4±0.9)mm]和EJ4[85日龄,壳长为(30.0±2.0)mm]的心率规律,进而探索EJ4心率对不同浓度Cd2+(0.0025、0.005、0.025、0.1和0.5 mg/L)的毒性响应特征。结果显示,早期幼蚌心率总体无显著日变化(P>0.05);心率与日龄和壳长之间均呈显著的线性负相关(P<0.05);心率和暴露时间仅在0.0025和0.5 mg/L的Cd^2+暴露组之间呈显著线性负相关(P<0.05);所有暴露组的心率和暴露浓度之间均呈显著线性负相关(P<0.05)。本研究表明,利用“标准化”背角无齿蚌幼蚌的心率对Cd^2+浓度的响应特征,有潜力开发出基于心率为指示的新型水质Cd^2+污染预警监测系统。This study investigates the response of heart rates to cadmium-related stress and its early warning capacity among juveniles of“standardized”Anodonta woodiana,a unique bioindicator used in the“Freshwater Mussel Watch”project.Heart rates of early juvenile mussels at several typical growth stages,from heartbeat-clearly-observed to shells-no-longer-transparent,i.e.,EJ1[25 days old;shell length(1.5±0.1)mm],EJ2[55 days old;shell length(10.5±0.6)mm],EJ3[70 days old;shell length(20.4±0.9)mm],and EJ4[85 days old;shell length(30.0±2.0)mm],were studied under non-lethal/non-injury conditions.The response of heart rates to different concentrations of Cd^2+(0.0025,0.005,0.025,0.1,and 0.5 mg/L)in EJ4 were examined.The results show that heart rates of early juveniles generally differed little between ante meridiem and post meridiem(P>0.05)states.Heart rates were linearly and negatively correlated with age and shell length(P<0.05).Heart rates were linearly and negatively correlated with exposure time in the exposure groups of 0.0025 and 0.5 mg/L Cd^2+(P<0.05).However,heart rates were linearly and negatively correlated with Cd^2+ concentrations in all exposure groups(P<0.05).These results indicate that a new early warning/monitoring system for Cd^2+ contamination may be developed using responses characteristic of“standardized”A.woodiana to Cd2+concentrations.

关 键 词：“标准化”背角无齿蚌 早期幼蚌 镉 心率 毒性响应 污染预警 

分 类 号：X171.5[环境科学与工程—环境科学]