中国拟青霉液体深层发酵条件的优化  

The Optimization of Liquid Submerged Fermentation Conditions of Paecilomyces sinensis

作　　者：周家萍[1,2] 孟梦 夏国强 ZHOU Jia-ping;MENG Meng;XIA Guo-qiang(Research Centre of Modern Analytical Technology,Tianjin University of Science&Technology,Tianjin 300457,China;State Key Laboratory of Food Nutrition and Safety,Tianjin University of Science&Technology,Tianjin 300457,China;College of Food Science and Engineering,Tianjin University of Science&Technology,Tianjin 300457,China;Youzifu(Tianjin)Food Technology Co.,Ltd.,Tianjin 300457,China)

机构地区：[1]天津科技大学现代分析技术研究中心,天津300457 [2]天津科技大学食品营养与安全国家重点实验室,天津300457 [3]天津科技大学食品科学学院,天津300457 [4]优滋福(天津)食品科技有限公司,天津300457

出　　处：《食品研究与开发》2021年第1期172-176,共5页Food Research and Development

摘　　要：中国拟青霉(Paecilomyces sinensis sp nov,PS)属于冬虫夏草中的一个无性型菌株,研究表明其与冬虫夏草在生物活性成分方面十分接近。该文通过单因素和正交试验对中国拟青霉液体深层发酵进行条件优化。对中国拟青霉进行发酵培养,选取菌丝体生物量为指标,确定碳源、氮源和无机盐的最佳比例和最优发酵条件。中国拟青霉液体深层发酵的最佳培养基配比为:玉米粉4.0%、葡萄糖1.5%、黄豆粉2.0%、酵母粉0.3%、KH2PO40.2%、MgSO40.1%、CaSO40.1%、ZnSO40.03%。优化后的菌丝体生物量比优化前提高了1.22倍,而胞内糖肽产量与优化前相比提高了54.0%。Paecilomyces sinensis sp nov is an anamorphic strain isolated from Cordyceps sinensis,which is traditional Chinese medicine.It exhibits high similarity to cordyceps sinensis in bioactive components.In this study,the conditions of submerged fermentation were optimized through single factor screening test and orthogonal experiments.The optimal ratio of carbon source,nitrogen source and inorganic salt was identified,accessing to the mycelia biomass.The optimal medium ratio of the submerged fermentation was as follows:corn flour 4.0%,glucose 1.5%,soybean meal 2.0%,yeast extrct 0.3%,KH2PO40.2%,MgSO40.1%,CaSO40.1%,ZnSO40.03%.The biomass of mycelium after optimization was 1.22 times higher than that before.The yield of intracellular glycopeptide was increased by 54.0%compared with that before.

关 键 词：中国拟青霉 液体发酵 生物量 正交试验 工艺优化 

分 类 号：TS201.3[轻工技术与工程—食品科学]