DC-CIK细胞对急性白血病细胞体外杀伤作用的研究  

Study on the Killing Effect of DC-CIK Cells on Acute Leukemia Cells in Vitro

作　　者：王俊[1] 向健[2] WANG Jun;XIANG Jian(Department of Clinical Laboratory,Tianmen First People's Hospital of Hubei Province,Tianmen Hubei 431700,China;Department of Blood Transfusion,Tianmen First People's Hospital of Hubei Province,Tianmen Hubei 431700,China)

机构地区：[1]湖北省天门市第一人民医院检验科,湖北天门431700 [2]湖北省天门市第一人民医院输血科,湖北天门431700

出　　处：《中国继续医学教育》2021年第1期126-130,共5页China Continuing Medical Education

摘　　要：目的探讨DC-CIK细胞对急性白血病细胞的体外杀伤作用。方法选取2017年9月—2019年4月本院收治的急性白血病患者98例,随机分为CIK组和DCCIK组各49例。选择健康人外周血单个核细胞诱导的CIK和DC细胞一同培养,将CIK细胞单独培养作为对照组。比较DC—CIK细胞免疫表型变化、DC-CIK细胞对白血病细胞的杀伤活性、DC-CIK细胞和CIK细胞分泌细胞因子水平。结果单独培养CIK细胞会随着时间的增加而导致CD3+CD56+、CD8+、CD3+双阳性细胞比例随之增加(P<0.05);CIK细胞和DC细胞一同培养时CD3+CD56+、CD8+、CD3+的双阳性细胞均高于同条件没有进行培养的CIK细胞和DC细胞组(P<0.05);培养后的DC-CIK细胞对ANLL细胞以及ALL细胞的杀伤活性均高于CIK细胞组(P<0.05),对ANLL、ALL的杀伤率差异无统计学意义(P>0.05);在40:1-5:1效靶中,效靶和杀伤作用呈现正相关(P<0.05);CIK细胞单独培养组IFN-γ、IL-12的分泌量低于DC-CIK细胞共同培养组(P<0.05)。结论DC-CIK细胞的抗急性白血病的活性以及增值能力都高于CIK细胞,DC-CIK细胞抵抗急性白血病治疗具有一定的作用。Objective To investigate the killing effect of DC-CIK cells on acute leukemia cells in vitro.Methods From September 2017 to April 2019,98 patients with acute leukemia admitted to our hospital were randomly divided into CIK group and DC-CIK group with 49 cases each.CIK and DC cells induced by healthy human peripheral blood mononuclear cells were cultured together,and CIK cells were cultured separately as control group.The immunophenotypic changes of DC-CIK cells,the killing activity of DC-CIK cells on leukemia cells,and the cytokine levels of DC-CIK cells and CIK cells were compared.Results The proportion of CD3+CD56+,CD8+and CD3+double positive cells increased with the increase of time in CIK cells alone P All of them were higher than that of CIK cell group(P<0.05),and there was no significant difference in the killing rate of ANLL and all(P>0.05);in the 40:1-5:1 target,there was a positive correlation between the target and killing effect(P<0.05);the secretion of IFN-γand IL-12 in CIK cell culture group was lower than that in DC-CIK cell co culture group(P<0.05).Conclusion DC-CIK cells have higher anti acute leukemia activity and value-added ability than CIK cells.DC-CIK cells have a certain role in anti acute leukemia treatment.

关 键 词：DC-CIK 急性白血病细胞 体外杀伤 杀伤活性 临床应用 研究 

分 类 号：R733[医药卫生—肿瘤]