玉米叶片表皮蜡质相关性状的全基因组关联分析  

Genome-wide Association Studies of Plant Epicuticular Waxes Trait in Maize

作　　者：王路林 闫鹏帅[1] 张稳 潘凯凯 张园园 白光红[2] 付志远[1] WANG Lu-lin;YAN Peng-shuai;ZHANG Wen;PAN Kai-kai;ZHANG Yuan-yuan;BAI Guang-hong;FU Zhi-yuan(College of Agronomy,Henan Agricultural University/National Key Laboratory of Wheat and Maize Crops Science,Department of Agronomy,Zhengzhou 450046;Xinjiang Agricultural University,Urumqi 830052,China)

机构地区：[1]河南农业大学农学院/国家小麦玉米作物学重点实验室,郑州450046 [2]新疆农业大学,乌鲁木齐830052

出　　处：《玉米科学》2020年第6期39-44,共6页Journal of Maize Sciences

基　　金：国家地区联合基金项目(U1604113和31760389);河南省高等学校重点科研项目(13A210469);小麦玉米作物学国家重点实验室自主设置课题(SKL2015ZH-01)。

摘　　要：为发掘玉米叶片表皮蜡质合成与调控相关基因,以218份由温带、亚热带和热带自交系组成的关联群体为材料,在原阳对其3个生物学重复叶片表皮挂水能力进行调查,利用1.25 M覆盖玉米全基因组的SNPs进行Q、K和Q+K这3种模型下的全基因组关联分析。结果表明,Q模型较K模型和Q+K模型能更好地评价叶片表皮的挂水能力。Q模型下,共检测到88个覆盖玉米9条染色体的显著SNPs(P≤2.04E-6),88个SNPs分布于47个QTLs内,单个QTL可解释13.6%~45.6%的叶片挂水能力表型变异,47个QTL内共有97个候选基因,其中,77个具有功能注释。位于第2染色体上的转录因子NAC77(GRMZM2G018436)和第3条染色体上的亚油酸酯氧合酶(GRMZM2G156861)编码基因,是叶片表皮蜡质性状的重要候选基因。To identify candidate genes controlling and regulating epicuticular wax biosynthesis,the association panel of 218 inbred lines composed with temperate,subtropical,and tropical materials were used.Their water-holding capacity was surveyed with three replications in Yuanyang.A genome-wide association study(GWAS)was carried out with 1.25 M SNPs under Q,K,and Q+K models,respectively.The Q model showed better in reducing type I errors(false positive)and evaluating epicuticular waxes than K model and Q+K model.Totally,88 significant association SNPs(P≤2.04 E-6)were detected on nine chromosomes,which was included in 47 non-redundant QTLs.Each QTL can explain 13.6%to 45.6%of the water-holding capacity variation.There were 97 candidate genes in these QTLs,and 77 out of them were annotated.NAC77 transcription factor(GRMZM2 G018436)on chromosome 2 and lipoxygenase(GRMZM2 G156861)on chromosome 3 were high lighted in this study as the preferred candidate genes for epicuticular wax.

关 键 词：玉米 全基因组关联分析 叶片表面蜡质 挂水能力 

分 类 号：S513.035.3[农业科学—作物学]