Vδ1 T细胞和TINs细胞表达情况与宫颈癌患者临床分期及预后的关系  

The relationship between the expression of Vδ1 T cells and TINs cells and the clinical stage and prognosis of cervical cancer patients

作　　者：张秀艳[1] 纪利娜[1] 梁紫影 孙海燕 刘东芳 ZHANG Xiuyan;JI Lina;LIANG Ziying;SUN Haiyan;LIU Dongfang(Department of Obstetrics and Gynecology,Chengde Central Hospital,Chengde 067000,China)

机构地区：[1]承德市中心医院妇产科,067000

出　　处：《免疫学杂志》2021年第1期67-72,共6页Immunological Journal

基　　金：承德市科技支撑计划(201701A019)。

摘　　要：目的探讨Vδ1 T细胞和肿瘤浸润性中性粒细胞(tumor infiltrating neutrophil,TINs)细胞表达情况及其与宫颈癌临床分期及预后关系。方法选取我院2012年1月至2017年1月期间经组织病理学确诊的60例宫颈癌患者为研究组,根据临床分期分为Ⅰ~Ⅳ期,患者数22例、18例、12例、8例,并选择同期100例良性病变者作为对照组。采用流式细胞术检测所有患者组织标本中Vδ1 T细胞表达水平,检测CD66b+TINs水平,IL^(-1)1、IL-8、IL^(-1)8炎性细胞因子,CCL2、CCL5、CXCL10趋化因子mRNA水平,并评价上述细胞因子表达水平与宫颈癌临床分期之间的相关性;采用ROC曲线评估Vδ1 T细胞和TINs细胞联合检测对宫颈癌患者预后预测价值。结果不同临床分期宫颈癌患者炎性细胞因子(IL^(-1)1、IL-8、IL^(-1)8)水平以及趋化因子(CCL2、CCL5、CXCL10)水平相比对照组均明显升高,且Ⅲ期和Ⅳ期患者高于Ⅰ期组患者(P<0.001);多元Logistic回归分析结果显示γδT细胞比例、CD66b+TINs百分比以及IL^(-1)1、IL-8、IL^(-1)8以及CCL2、CCL5均属于影响宫颈癌患者临床分期的危险因素(P<0.05);随访3年,生存率为68.33%(41/60),ROC曲线分析结果显示联合诊断曲线下面积为0.895(95%CI:0.795~0.965),灵敏度为84.2%,特异度73.2%,约登指数为0.574,具有最佳诊断效能。结论宫颈癌患者Vδ1 T细胞和TINs细胞随着临床分期进展而升高,联合检测对于宫颈癌患者预后具有一定的预测价值。To investigate the relationship between the expression of Vδ1 T cells and tumor infiltrating neutrophil(TINs)cells and the clinical stage and prognosis of cervical cancer patients,60 patients with cervical cancer diagnosed by histopathology from January 2012 to January 2017 in our hospital were selected as the research group.According to the clinical T stage,the enrolled patients were divided into stages ofⅠ-Ⅳwith 22 cases,18 cases,12 cases,and 8 cases in each stage.And 100 cases of benign lesions in the same period were selected as the control group.The expression levels of Vδ1 T cells,CD66b+TINs,IL^(-1)1,IL-8,CCL2,CCL5,CXCL10 were detected and the correlation between the above cytokine expression levels and the clinical stage of cervical cancer were evaluated;ROC curve was used to evaluate the predictive value of the combined detection of Vδ1 T cells and TINs cells in the prognosis of cervical cancer patients.Data showed that the levels of inflammatory cytokines(IL^(-1)1,IL-8,IL^(-1)8)and chemokines(CCL2,CCL5,CXCL10)in cervical cancer patients with different clinical stages were significantly higher than those in the control group,and patients in stagesⅢand IV were higher than those in stageⅠ(P<0.001);The results of multiple logistic regression analysis showed that the ratio ofγδT cells,the percentage of CD66b+TINs,and the levels of IL^(-1)1,IL-8,IL^(-1)8,CCL2 and CCL5 were all risk factors affecting the clinical stage of cervical cancer patients(P<0.05).After 3 years of follow-up,the survival rate of cervical cancer patients was 68.33%(41/60).The results of ROC curve analysis showed that the area under the combined diagnosis curve was 0.895(95%CI:0.795~0.965),the sensitivity was 84.2%,the specificity was 73.2%,and the index was 0.574,with the best diagnostic performance.In conclusion,the levels of Vδ1 T cells and TINs cells are increased in cervical cancer patients with the increase of clinical stage,and the combined detection has certain predictive value for the prognosis of cervical cancer pati

关 键 词：Vδ1 T细胞 肿瘤浸润性中性粒细胞 宫颈癌 临床分期 

分 类 号：R737.33[医药卫生—肿瘤]