鼻咽癌组织中BMAL1和CerbB-2基因的表达及机制  

Expressions of BMAL1 and CerbB-2 genes in nasopharyngeal carcinoma

作　　者：王波涛[1] 赵琳[1] 夏翠[1] 祝康[1] 高天喜[1] 喻超 孙斌[1] WANG Botao;ZHAO Lin;XIA Cui;ZHU Kang;GAO Tianxi;YU Chao;SUN Bin(Department of Otolaryngology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710004, China)

机构地区：[1]西安交通大学第二附属医院耳鼻喉科,陕西西安710004

出　　处：《西安交通大学学报（医学版）》2021年第1期65-69,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：陕西省教育厅专项科研计划项目(No.12JK0773)。

摘　　要：目的探讨鼻咽癌组织中BMAL1以及CerbB-2基因的表达及相关机制。方法用质粒转染、MTT、RT-PCR和蛋白免疫印迹方法分别检测鼻咽癌细胞的增殖、BMAL1和CerbB-2基因及蛋白的表达,以及NF-κB信号通路中p50和p65的表达。结果MTT实验结果表明,BMAL1和CerbB-2影响鼻咽癌细胞的增殖;RT-PCR实验结果显示,BMAL1 mRNA在鼻咽癌中明显下调(CK:2.24±0.22,NPC:0.63±0.11,P<0.01),而CerbB-2 mRNA的表达则明显上调(CK:0.89±0.13,NPC:2.65±0.25,P<0.01)。NF-κB信号通路中,p50和p65 mRNA在NPC细胞中均表现上调的趋势[p50:CK(0.48±0.12),NPC(1.45±0.25);p65:CK(0.52±0.12),NPC(2.33±0.35),P<0.01];蛋白免疫印迹结果与mRNA表达情况一致,进一步证实了研究结果的可靠性。结论BMAL1和CerbB-2通过炎症通路NF-κB调节鼻咽癌细胞的侵袭和转移的分子机制,为鼻咽癌的治疗和预防提供了进一步的理论支持。Objective To study the expressions of BMAL1 and CerbB-2 genes in nasopharyngeal carcinoma(NPC)and their related mechanisms.Methods Plasmid transfection,MTT,RT-PCR and Western blotting were used to detect the proliferation of NPC cells,the expressions of BMAL1 and CerbB-2 genes and proteins,and the expression of NF-κB signaling pathway.Results The MTT results showed that BMAL1 and CerbB-2 could affect the proliferation of NPC cells.RT-PCR results showed that BMAL1 gene was significantly down-regulated in NPC(CK:2.24±0.22,NPC:0.63±0.11,P<0.01),while CerbB-2 gene expression was significantly up-regulated(CK:0.89±0.13,NPC:2.65±0.25,P<0.01).The p50 and p65 genes in the NF-κB signaling pathway were up-regulated in NPC cells(p50,CK:0.48±0.12,NPC:1.45±0.25;p65,CK:0.52±0.12,NPC:2.33±0.35,P<0.01).The results of Western blotting were consistent with gene expression,which further confirmed the reliability of the results.Conclusion This study reveals that BMAL1 and CerbB-2 regulate the invasion and metastasis of NPC cells through the inflammatory pathway NF-κB,providing further theoretical support for the treatment and prevention of NPC.

关 键 词：鼻咽癌 NF-ΚB信号通路 BMAL1 CERBB-2 

分 类 号：R541.4[医药卫生—心血管疾病]