三株美洲驼源狂犬病病毒N基因遗传进化分析  

Genetic Evolution Analysis of N Genes of Three Strains of Rabies Viruses Isolated from Llamas(Lama glama)

作　　者：王金玲[1,2] 郑秉武 曹霞 杨龙峰 葛金英 卢爱桃[6] WANG Jinling;ZHENG Bingwu;CAO Xia;YANG Longfeng;GE Jinying;LU Aitao(College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot,010010,China;Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease,Ministry of Agriculture and Rural Affairs,Hohhot,010010,China;Zoo Management Office,Hohhot Landscape Bureau,Hohhot,010050,China;Beijing Yanqing District Center for Animal Disease Control and Prevention,Beijing,102100,China;State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin,150001,China;Inner Mongolia Comprehensive Center for Disease Control and Prevention,Hohhot,010031,China)

机构地区：[1]内蒙古农业大学兽医学院,呼和浩特010010 [2]农业农村部动物疾病临床诊疗技术重点实验室,呼和浩特010010 [3]呼和浩特市园林管理局动物园管理处,呼和浩特010050 [4]北京市延庆区动物疫病预防控制中心,北京102100 [5]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,哈尔滨150001 [6]内蒙古自治区综合疾病预防控制中心,呼和浩特010031

出　　处：《野生动物学报》2021年第1期120-123,共4页CHINESE JOURNAL OF WILDLIFE

基　　金：内蒙古自治区高等学校科学研究项目(NJZY17073)。

摘　　要：为阐明2015年在内蒙古呼和浩特地区3株美洲驼源狂犬病病毒(RABV)流行株的遗传进化情况,经基因扩增和测序获得3株美洲驼源RABV N基因序列,并进行遗传进化分析。结果显示3株RABV之间N基因核苷酸序列和氨基酸序列同源性均为100%,且都与2011年呼和浩特奶牛源和犬源毒株、2013年内蒙古牛源毒株、2007年湖北犬源毒株、2016和2018年北京犬源毒株、2006年天津犬源毒株和2009年福建犬源毒株的亲缘性最高,同源率为100%。这些毒株位于同一个遗传进化群内,均属于CTN-1(Asian谱系)。研究结果说明3株美洲驼源RABV的N基因与参考的内蒙古、湖北、北京、福建和天津毒株起源于共同的祖先。To elucidate the genetic evolution of 3 strains of llama-derived rabies virus(RABV)circulating in Inner Mongolia in 2015,the N gene sequences of 3 isolates were obtained by gene amplification and sequencing,and genetic evolution analysis was conducted.Sequence analysis showed that the nucleotide similarities of N genes of the 3 isolates were 100%.The N genes of the 3 isolates had the highest sequence identity(100%)with the RABV from:(1)cattle(Bos taurus)in Hohhot in 2011 and Inner Mongolia in 2013;(2)dogs(Canis lupus familiaris)in Hohhot in 2011,Hubei in 2007,Beijing in 2016 and 2018,Tianjin in 2006,and Fujian in 2009.These N genes belonged to the CTN-1(Asian lineage).The results indicated that the N genes of the 3 isolates and the referenced strains in Inner Mongolia,Hubei,Beijing,Tianjin and Fujian stem from a common ancestor.

关 键 词：美洲驼 狂犬病病毒 N基因 遗传进化 

分 类 号：S852.65+9.5[农业科学—基础兽医学]