酸性稻田土n-damo细菌的多样性和丰度  

Diversity and Abundance of Nitrite Dependent Anaerobic Methane Oxidizer in Acidic Paddy Soil

作　　者：常佳丽 张元勤[1] 江滔[1] 马旭光[1] CHANG Jiali;ZHANG Yuanqin;JIANG Tao;MA Xuguang(School of Chemistry,Resources and Environment,Leshan Normal University,Leshan Sichuan 614000,China)

机构地区：[1]乐山师范学院化学与资源环境学院,四川乐山614000

出　　处：《乐山师范学院学报》2020年第12期33-38,共6页Journal of Leshan Normal University

基　　金：乐山师范学院引进人才科研启动项目“反硝化型甲烷厌氧氧化过程的电生物强化及机理研究”(XJR18004);国家青年科学基金项目“微好氧菌群强化分解木质纤维素及其提高甲烷产量的机理研究”(51508258)。

摘　　要：亚硝酸盐依赖型甲烷厌氧氧化(n-damo)细菌是新近发现的一类具有特殊功能的微生物,在废水深度脱氮领域具有潜在应用前景。研究利用n-damo菌16S rRNA和pmoA基因的专一性引物,通过系统发育分析和定量PCR技术,检测酸性稻田土中该类群的多样性和丰度。结果显示,在弱酸性中等养分水平的稻田土中,n-damo菌聚为3~4个OTUs类群;系统发育分析表明,酸性稻田土中存在新型未知n-damo类群;n-damo细菌丰度较高,16S rRNA基因拷贝数可达1.25~2.31×10^8拷贝每克干重,pmoA基因拷贝数为8.66×10^6~2.54×10^7拷贝每克干重。研究为后续富集培养嗜酸型n-damo菌提供可靠菌源。The recently discovered nitrite-dependent anaerobic methane oxidizer(n-damo)has a promising application capability in the field of nitrogen removal of wastewater.This study focuses on detecting the diversity and abundance of n-damo bacteria in acidic paddy soil,using phylogenetic analysis and quantitative polymerase chain reaction technology based on n-damo-specific 16S rRNA gene and pmoA gene.The results show that n-damo bacteria clusters into 3-4 OTUs in the paddy soil samples with weak acidity and moderate nutrient level.Phylogenetic analysis indicates that novel unknown n-damo bacteria exist in the acidic paddy soil.The abundance of n-damo bacteria is generally high,with 16S rRNA gene and pmoA gene in the range of 1.25~2.31×10^8 and 8.66×10^6~2.54×10^7 copies per g dry weight,respectively.Our study offers reliable source of inoculum for subsequent enrichment of acidophilic n-damo bacteria.

关 键 词：亚硝酸盐依赖型甲烷厌氧氧化细菌 酸性稻田土 系统发育分析 定量PCR 

分 类 号：X172[环境科学与工程—环境科学]